COVID-19

The Krammer Laboratory are currently working to provide reagents and standardized protocols for COVID-19 testing.

Several of the expression plasmids and proteins have been submitted to BEI Resources and can be requested from their web page for free (https://www.beiresources.org/) or plasmids and proteins can be requested from this form https://forms.gle/x6XT7yWg5yE5WC9Q7. For commercial requests, please email Susie Dong at changsu.dong@mssm.edu.

Expression Plasmids

  • Soluble SARS-CoV-2 Receptor Binding Domain cloned in mammalian expression vectors
  • Soluble SARS-CoV-2 Receptor Binding Domain cloned in baculovirus transfer vectors NOT AVAILABLE
  • Soluble SARS-CoV-2 Spike cloned in mammalian expression vectors
  • Soluble SARS-CoV-2 Spike cloned in baculovirus transfer vectors NOT AVAILABLE
  • SARS-CoV-2 Spike with a transmembrane domain cloned in mammalian expression vectors

Recombinant Proteins

  • Soluble SARS-CoV-2 Receptor Binding Domain expressed in mammalian cells
  • Soluble SARS-CoV-2 Receptor Binding Domain expressed in the baculovirus system NOT AVAILABLE
  • Soluble SARS-CoV-2 Spike protein expressed in mammalian cells
  • Soluble SARS-CoV-2 Spike protein expressed in the baculovirus system NOT AVAILABLE
  • SARS-CoV-2 Spike with a transmembrane domain expressed in mammalian cells NOT AVAILABLE

Protocols

A detailed protocol describing antigen expression for immunological testing using our expression plasmids and ELISA setup can be downloaded here Stadlbauer and Amanat COVID19 antigen expression and serology protocol 3_24_20

COVID-19 Support

For donations to support the Krammer Laboratory’s work on COVID-19 please email Vanesa Sarić (vanesa.saric@mountsinai.org) from Mount Sinai Development.