Giuseppe Pisanelli, D.V.M., Ph.D.





Research Giuseppe



Research Interests
Yellow fever virus (YFV) is the etiologic agent of yellow fever. There are no antivirals available to treat yellow fever, and attempts to develop YFV-specific antivirals or to develop alternative vaccines have been hampered by the lack of an immunocompetent mouse model of yellow fever. It has been reported that both the wild-type (Asibi) strain and the 17D strain of yellow fever infect mice with defects in type I IFN (IFN-I) signaling. This suggests that the IFN-I response serves as the major barrier to mouse infection. We have recently shown that YFV antagonizes IFN-I signaling in human and non-human primate cells and that the NS5 proteins of both YFV-Asibi and YFV-17D bind to and sequester human STAT2 after interferon signaling. My research project’s aim is to identify the host restriction factors responsible for the inability of the YF-NS5 protein to bind to murine STAT2 and inhibit murine IFN-I signaling. This will be potentially useful for the development of a Yellow Fever mouse model.

Notable Publications
Giuseppe Pisanelli, Maudry Laurent-Rolle, Balaji Manicassamy, Alan Belicha-Villanueva, Juliet Morrison, Bernardo Lozano-Dubernard, Felipa Castro-Peralta, Giuseppe Iovane, and Adolfo García-Sastre. La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation. Virus Research. 2016 Feb 2;213:11-22. doi: 10.1016/j.virusres.2015.10.027.

Maudry Laurent-Rolle, Juliet Morrison, Ricardo Rajsbaum, Jesica M. Levingston Macleod, Giuseppe Pisanelli, Alissa Pham, Juan Ayllon, Lisa Miorin, Carles Martínez-Romero, Benjamin R Tenoever, and Adolfo García-Sastre. The interferon signaling antagonist function of yellow fever virus NS5 protein is activated by Type I interferon. Cell Host and Microbes. 2014 Sep 10;16(3):314-27. doi: 10.1016/j.chom.2014.07.015.

Ricardo Rajsbaum, Gijs A. Versteeg, Sonja Schmid, Ana M. Maestre, Alan Belicha-Villanueva, Carles Martínez-Romero, Jenish R. Patel, Juliet Morrison, Giuseppe Pisanelli, Lisa Miorin, Maudry Laurent-Rolle, Hong M. Moulton, David A. Stein, Ana Fernandez-Sesma, Benjamin R. Tenoever, and Adolfo García-Sastre. Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response. Immunity. 2014 Jun 19;40(6):880-95. doi: 10.1016/j.immuni.2014.04.018. Epub 2014 May 29. PMID: 24882218.

Juliet Morrison, Maudry Laurent-Rolle, Ana Maestre, Giuseppe Pisanelli, Lubbertus C.F. Mulder, Viviana Simon, Ana Fernandez-Sesma, Adolfo García-Sastre. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I IFN signaling. PLOS Pathogens. 2013 Mar;9(3):e1003265. doi: 10.1371/journal.ppat.1003265. Epub 2013 Mar 28.

Balaji Manicassamy, Santhakumar Manicassamy, Alan Belicha-Villanueva, Giuseppe Pisanelli, Bali Pulendran And Adolfo García-Sastre. Analysis of in vivo dynamics of influenza virus infection using a GFP reporter virus. Proceedings of National Academy of Sciences of  the United States of America. 2010 Jun 22;107(25):11531-6. Epub 2010 Jun 7.