The Friedman Laboratory in the Icahn Medical Institute Building at Mount Sinai is 1700 square feet. This space consists of:
- an open, modular design with ample natural light
- one seminar room
- 1100 square foot general area with 10 working benches, fume hood, and storage
- three dedicated tissue culture rooms, with culture hoods, incubators, and a perfusion area for primary liver cell isolation
- shared access on the floor to an autoclave and dishwashing room
- 4o C and -20o C walk-in cold rooms
- Vibratome tissue slicer (VT1200S. Leica) to generate precision cut liver slices
- tissue slice device for precision-cut liver slices
- Biotek Epoch Microplate Spectrophotometer, Biotek Synergy HT microplate reader, and a Milli-Q Academic water purifying system
- two dedicated –80o C freezers are available to the laboratory
- two liquid nitrogen tanks for human liver and mRNA storage, with a duplicate liver tissue bank inventory in each tank
- benchtop inverted microscopes for tissue and cell culture analysis are on-site
- Zeiss Axio Observer 7 Inverted Brightfield Microscope
Additional key equipment includes:
- Two dedicated Roche Light Cycler 480 for real time PCR
- A QiaCube automatic sample prep machine
- Veritas Luminometer
- Qiagen Tissue Lyser LT
- Life Technologies Countess cell counter
- Millipore Snap I.D. version 2
Each student/postdoc/technician bench is equipped with a full range of micropipetters, and a dedicated microcentrifuge and immunoblot apparatus.