Samuel Sidi, PhD
Developmental & Regenerative Biology
Medicine (Division of Hematology and Medical Oncology)
Ando, William Kiyohiro
I am focusing on the mechanism of caspase-2 activation in the Chk1-suppressed apoptotic pathway. I am also working on a genome-wide siena screen for new pathway components.
Zebrafish Facility Manager
Di Giandomenico, Silvana
My research seeks to define the genetic determinants of tumor cell response to radiation plus Chk1 inhibition. I am screening large panels of genotypical annotated cancer cell lines for their ability to activate caspase-2 after treatment. Candidate predictive genotypes are then validated in zebrafish, ex vivo patient cultures, xenograft models.
I am interested in the molecular events that lead to the execution of the Chk1-suppressed apoptotic program downstream of caspase-2 activation. Specifically, I am searching for regulators and substrates of this caspase using genetic and proteomic approaches.
I seek to find novel compounds that are synthetically lethal to p53 deficiency using zebrafish as a high-throughput screening system for small-molecule inhibitors.
My work focuses on the mechanism by which Chk1 negatively regulates PIDDosome assembly and activation in the Chk1-suppressed apoptotic pathway. I am also using a genome-wide siRNA screen to identify new pathway components.