Publications

  1. Tan, P., Fuchs, S.Y., Chen, A., Wu, K., Gomez, C., Ronai, Z., and Pan, Z.-Q. (1999) Recruitment of a ROC1:Cullin1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of IkBa. Mol. Cell 3, 527-533
  1. Wu, K., Fuchs, S.Y., Chen, A., Tan, P., Gomez, C., Ronai, Z., and Pan, Z.-Q. (2000) The SCFHOS/β-TRCP-ROC1 E3 Ubiquitin Ligase Utilizes Two Distinct Domains within CUL1 for Substrate Targeting and Ubiquitin Ligation. Mol. Cell. Biol. 20, 1382-1393
  1. Chen, A., Wu, K., Fuchs, S.Y., Tan, P., Gomez, C., and Pan, Z.-Q. (2000) The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation. J. Biol. Chem. 275, 15432-15439
  1. Wu, K, Chen, A, and Pan, Z.-Q. (2000) Conjugation of Nedd8 to CUL1 enhances the ability of the ROC1-CUL1 complex to promote ubiquitin polymerization. J Biol Chem. 275, 32317-32324
  1. Wu K, Chen A, Tan P, Pan, Z.-Q. (2002) The Nedd8-conjugated ROC1-CUL1 Core Ubiquitin Ligase Utilizes Nedd8 Charged Surface Residues For Efficient Polyubiquitin Chain Assembly Catalyzed By Cdc34. J Biol Chem 277: 516-527.
  1. Chen A, Kleiman FE, Manley JL, Ouchi T, Pan Z-Q. (2002) Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase. J Biol Chem 277(24): 22085-92.
  1. Dias, DC, Dolios, G, Wang, R., Pan, Z-Q. (2002) CUL7: a DOC domain-containing cullin selectively binds Skp1•Fbx29 to form a novel SCF-like complex. Proc. Natl. Acad. Sci. USA, 99(26): 16601-6
  1. Wu K, Yamoah K, Dolios G, Gan-Erdene T, Tan P, Chen A, Lee CG, Wei N, Wilkinson KD, Wang R, Pan ZQ. (2003) DEN1 is a dual function protease capable of processing the C-terminus of Nedd8 and deconjugating hyper-neddylated CUL1. J Biol. Chem., 278, 28882-91
  1. Pan ZQ, Kentsis A, Dias DC, Yamoah K., and Wu K. (2004). Nedd8 on cullin: building an expressway to protein destruction. Oncogene, 23: 1985-1997.
  1. Miller F, Kentsis A, Osman R, and Pan ZQ. (2005) Inactivation of VHL by Tumorigenic Mutations that Disrupt Dynamic Coupling of the pVHL:HIF-1α Complex. J. Biol. Chem., 280: 7985-7996.
  1. Yamoah, K., Wu, K. and Pan ZQ, (2005). Analysis of deneddylation activities catalyzed by DEN1 and CSN in vitro. Methods in Enzymology, 398: 509-522.
  1. Huber C, Dias-Santagata D, Glaser A, O’Sullivan J, Brauner R, Wu K, Pearce K, Wang R, Luisa M, Uzielli G, Dagoneau N, Chemaitilly W, Superti-Furga A, Dos Santos H, Mégarbané A, Morin G, Gillessen-Kaesbach G, Hennekam R, Brunner H, Graeme Black GCM, Clayton PE, Read A, Le Merrer M, Scambler PJ, Munnich A, Pan ZQ, Winter R, and Cormier-Daire V. (2005) Identification of CUL7 mutations in the 3-M syndrome. Nature Genetics, 37: 1119-1124
  1. Gazdoiu, S, Yamoah, K, Wu, K, Escalante, CR, Tappin, I, Bermudez, V, Aggarwal, AK, Hurwitz, J, and Pan, ZQ. (2005) Proximity-induced activation of human Cdc34 through heterologous dimerization, Proc. Natl. Acad. Sci. USA, 102: 15053-15058
  1. Gazdoiu S, Yamoah K, Wu K, Pan ZQ. (2007). Human Cdc34 employs distinct sites to coordinate attachment of ubiquitin to a substrate and assembly of polyubiquitin chains. Mol Cell Biol. 27(20): 7041-52.
  1. Xu X, Sarikas A, Dias-Santagata DC, Dolios G, Lafontant PJ, Tsai SC, Zhu W, Nakajima H, Nakajima HO, Field LJ, Wang R, and Pan ZQ. (2008) The CUL7 E3 ubiquitin ligase targets insulin receptor substrate 1 for ubiquitin-dependent degradation. Mol. Cell, 30: 403-414.
  1. Sarikas A, Xu X, Field LJ, and Pan ZQ. (2008) The Cullin7 E3 ubiquitin ligase: a novel player in growth control. Cell Cycle 7(20): 3154-61.
  1. Yamoah Y, Oashi T, Sarikas A, Gazdoiu S, Osman R, and Pan ZQ. (2008) Auto-inhibitory regulation of SCF-mediated ubiquitination by human cullin 1’s C-terminal tail. PNAS, 105(34): 12230-5.
  1. Wu K, Kovacev J, and Pan ZQ (2010) Priming and extending: an UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate. Mol. Cell 37: 784-796.
  1. Choi YS, Wu K, Jeong K, Lee D, Jeon YH, Choi BS, *Pan ZQ, *Ryu KS, *Cheong C. (2010) The human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination. J Biol Chem. 285(23): 17754-62. (*Co-corresponding authors)
  1. Wu K, Yan H, Fang L, Wang X, Pfleger C, Jiang X, Huang L, Pan ZQ. (2011) Mono-ubiquitination drives nuclear export of the human Dcn1-like protein hDCNL1. J Biol Chem. 286(39): 34060-70
  1. Sarikas A, Hartmann T, and Pan ZQ. (2011) The cullin protein family. Genome Biology 12(4): 220.
  1. Xu X, Keshwani M, Meyer K, Sarikas A, Taylor S, Pan ZQ. (2012) Identification of insulin receptor substrate 1’s degradation determinants for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination. J Biol Chem. 287(48): 40758-662012. PMID: 23045529; PMCID: PMC3504788
  1. Kovacev J, Wu K, Spratt DE, Chong RA, Lee C, Nayak J, Shaw GS, Pan ZQ. (2014) A snapshot at ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination. J Biol Chem. 289(10): 7068-81.
  1. Chong RA, Wu K, Spratt DE, Yang Y, Lee L, Nayak J, Xu M, Elkholi R, Tappin I, Li J, Hurwitz J, Brown BD, Chipuk JE, Chen Z, Sanchez R, Shaw GS, Huang L and Pan ZQ. (2014) Pivotal role for the ubiquitin Y59-E51 loop in lysine 48 polyubiquitination. PNAS, 111(23): 8434-9.
  1. Chong RA, Wu K, Kovacev J, and Pan ZQ (2015) Generation of a proteolytic signal: E3/E2-mediated polyubiquitination of IκBα. Methods in Molecular Biology (Humana Press), Volume of “NF-κB: Methods and Protocols.”
  1. Wu K, Chong RA, Yu Q, Bai J, Spratt DE, Ching K, Lee C, Miao H, Tappin I, Jerard Hurwitz J, Zheng N, Shaw GS, Sun Y, Felsenfeld DP, Sanchez R, Zheng J, and Pan ZQ. (2016) Suramin inhibits cullin-RING E3 ubiquitin ligases. Proc Natl Acad Sci U S A. Mar 21. pii: 201601089. [Epub ahead of print] PMID: 27001857