Skobe Laboratory


Gibot L, Galbraith T, Bourland J, Rogic A, Skobe M, Auger FA. Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis. Nature Protocols 2017; 12:1077-1088. Article Link

Gibot L, Galbraith T, Kloos B, Das S, Lacroix DA, Auger FA, Skobe M. Cell-based approach for 3D reconstruction of lymphatic capillaries in vitro reveals distinct functions of HGF and VEGF-C in lymphangiogenesis. Biomaterials 2015; 129-39. Article Link

Serasinghe MN, Missert DJ, Asciolla JJ, Podgrabinska S, Wieder SY, Izadmehr S, Belbin G, Skobe M, Chipuk JE. Anti-apoptotic BCL-2 proteins govern cellular outcome following B-RAFV600E inhibition and can be targeted to reduce resistance. Oncogene 2014; 1-11. Article Link

Zhao Y, van Rooy I, Hak S, Fay F, Tang J, Davies CL, Skobe M, Fisher EA, Radu A, Fayad ZA, de Mello DC, Meijerink A, Mulder WJ. Near-infrared fluorescence evergy transfer imaging of nanoparticle accumulation and dissociation kinetics in tumor-bearing mice. ACS Nano. 2013; 7:10362-70. Article Link

Das S, Sarrou E, Cassella M, Feirt N, Skobe M. Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses. J. Exp. Med. 2013; 210:1509-28. Article Link

Das S, Ladell DS, Podgrabinska S, Ponomarev V, Nagi C, Fallon JT, Skobe M. VEGF-C induces lymphangitic carcinomatosis, an extremely aggressive form of lung metastases. Cancer Res. 2010; 70:1814-24. Article Link

Lederle W, Linde N, Heusel J, Bzyl J, Woenne EC, Zwick S, Skobe M, Kiessling F, Fusenig NE, Mueller MM. Platelet-derived growth factor-B normalizes micromorphology and vessel Function in VEGF-A-induced squamous cell carcinomas. Am. J. Pathol. 2010; 176:981-94. Article Link

Podgrabinska S, Kamalu O, Mayer L, Shimaoka M, Randolph GJ, Skobe M. Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism. J. Immunol. 2009; 183:1767-79. Article Link

Shawber, CJ, Funahashi Y, Francisco E, Voronchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J. Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression. J. Clin. Inves.  2007; 117:3369-82. Article Link

Furtado GC, Marinkovic T, Martin AP, Garin A, Hoch B, Hubner W, Chen BK, Genden E, Skobe M, Lira SA. Lymphotoxin beta receptor signaling is required for inflammatory lymphangiogenesis in the thyroid. Proc. Natl. Acad. Sci. USA. 2007; 104:5026-31. Article Link

Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells. Am. J. Pathol. 2006; 169:1767-83. Article Link

Grossmann C, Podgrabinska S, SkobeM, Ganem D. Activation of NF-kappaB by the latent vFLIP gene of Kaposi’s sarcoma-associated herpesvirus is required for the spindle shape of virus-infected endothelial cells and contributes to their proinflammatory phenotype. J. Virol. 2006; 80:7179-85. Article Link

Roberts N, Kloos B, Cassella M, Podgrabinska S, PersaudK, WuY, Pytowski B, Skobe M. Inhibition of VEGFR-3 activation with the antagonistic antibody more potently suppresses lymph node and distant metastases than inactivation of VEGFR-2. Cancer Res. 2006; 66:2650-7. Article Link

Angeli V, Ginhoux F, Llodra J, Quemeneur L, Frenette PS, Skobe M, Jessberger R, Merad M, Randolph GJ. B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity 2006; 24:203-15. Article Link

Goldman J, Lee TX, Skobe M, Swartz M. Overexpression of VEGF-C causes transient lymphatic hyperplasia but not increased lymphangiogenesis in regenerating skin. Circ. Res. 2005; 96:1193-9. Article Link

Pytowski B, Goldman J, Persaud K, Wu Y, Witte L, Hicklin DJ, Skobe M, Boardman KC, Swartz M. Complete and specific inhibition of adult lymphatic regeneration by a novel VEGFR-3 neutralizing antibody. JNCI 2005; 97:14-21. Article Link

Sugaya M, Watanabe T, Yang A, Starost MF, Kobayashi H, Atkins AM, Borris DL, Hanan EA, Schimel D, Bryant MA, Roberts N, Skobe M, Staskus KA, Kaldis P, Blauvelt A. Lymphatic dysfunction in transgenic mice expressing KSHV k-cyclin under the control of the VEGFR-3 promoter. Blood 2005; 105:2356-63. Article Link

Gruss CJ, Satyamoorthy K, Berking C, Lininger J, Nesbit M, Schaider H, Liu ZJ, Oka M, Hsu MY, Shirakawa T, Li G, Bogenrieder T, Carmeliet P, El-Deiry WS, Eck SL, Rao JS, Baker AH, Bennet JT, Crombleholme TM, Velazquez O, Karmacharya J, Margolis DJ, Wilson JM, Detmar M, Skobe M, Robbins PD, Buck C, Herlyn M. Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice. J. Invest. Dermatol. 2003; 120:683-92. Article Link

Podgrabinska S, Braun P, Velasco P, Kloos B, Pepper MS, Jackson DG Skobe M. Molecular characterization of lymphatic endothelial cells. Proc. Natl. Acad. Sci. USA 2002; 99:16069-16074. Article Link

Szuba A, Skobe M, Karkkainen MJ, Shin WS, Beyner DP, Rockson NB, Dakhil N, Spilman S, Goris ML, Strauss HW, Quartermous T, Alitalo K, Rockson S. Therapeutic lymphangiogenesis with human recombinant VEGF-C. FASEB J. 2002; 16:1985-7. Article Link

Hawighorst T, Skobe M, Streit M, Hong Y-K, Velasco P, Brown LF, Riccardi L, Lange-Asschenfeldt B, Detmar M. Activation of the Tie2 receptor by angiopoietin-1 enhances tumor vessel maturation and impairs squamous cell carcinoma growth. Am. J. Pathol. 2002; 160:1381-92. Article Link

Skobe M, Hamberg L, Hawighorst T, Schirner M, Wolf GL, Alitalo K, Detmar M. Concurrent induction of lymphangiogenesis, angiogenesis and macrophage recruitment by VEGF-C in melanoma. Am. J. Pathol. 2001; 159:893-903. Article Link

Skobe M, Hawighorst T, Jackson D, Prevo R, Janes L, Velasco P, Riccardi L, Alitalo K, Claffey K, Detmar M. Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis. Nature Med. 2001; 7:192-198. Article Link

Detmar M, Velasco P, Richard L, Claffey KP, Streit M, Riccardi L, Skobe M, Brown LF. Expression of vascular endothelial growth factor induces an invasive phenotype in human squamous cell carcinomas. Am. J. Pathol. 2000; 156:159-67. Article Link

Skobe M, Brown LF, Tognazzi K, Ganju RK, Dezube BJ, Alitalo K, Detmar M. Vascular endothelial growth factor-C (VEGF-C) and its receptors KDR and flt-4 are expressed in AIDS-associated Kaposi’s sarcoma. J. Invest. Dermatol. 1999; 113:1047-53. Article Link

Streit M, Velasco P, Brown LF, Skobe M, Richard L, Riccardi L, Lawler J, Detmar M. Overexpression of thrombospondin-1 decreases angiogenesis and inhibits the growth of human cutaneous squamous cell carcinomas. Am. J. Pathol. 1999; 155:441-52. Article Link

Bajou K, Noel A, Gerard RD, Masson V, Brunner N, Holst-Hansen C, Skobe M, Fusenig NE, Carmeliet P, Collen D, Foidart JM. Absence of the host plasminogen activator inhibitor 1 prevents cancer invasion and vascularization. Nature Med. 1998; 4:923-928. Article Link

Skobe M, Fusenig NE. Tumorigenic conversion of immortal human keratinocytes through stromal cell activation. Proc. Natl. Acad. Sci. USA 1998; 95:1050-1055. Article Link

Skobe M, Vosseler S, Rockwell P, Goldstein N, Fusenig NE. Halting angiogenesis suppresses carcinoma cell invasion. Nature Med. 1997; 3:1222-1227. Article Link

Other Peer Reviewed Publications and Invited Contributions

Podgrabinska S, Skobe M. Role of lymphatic vasculature in regional and distant metastases. Microvasc. Res. 2014; 95:46-52. Article Link

Skobe M. Lymphatic vessels and cancer. Lymphat. Res. Biol. 2012; 10:96. Editorial. Article Link

Skobe M, Dana R. Blocking the path of lymphatic vessels. Nature Med. 2009; 15:993-4. Commentary. Article Link

Das S, Skobe M. Lymphatic vessel activation in cancer. Ann. N. Y. Acad. Sci. 2008; 1131:235-41. Review. Article Link

Rafii S, Skobe M. Splitting vessels: Keeping lymph apart from blood. Nature Med. 2003; 9:166-8. Commentary. Article Link

Cassella M, Skobe M. Lymphatic vessel activation in cancer. Ann. N. Y. Acad. Sci. 2003; 979:120-130. Review. Article Link

Pepper MS, Tille J-C, Nisato R, Skobe M. Lymphangiogenesis and tumor metastasis. Cell Tissue Res. 2003; 314:167-177. Review. (special issue dedicated to vascular biology). Article Link

Pepper MS, Skobe M. Lymphatic endothelium: Morphological, molecular and functional properties. J. Cell Bio. 2003; 163:209-13. Review. Article Link

Swartz MA, Skobe M. Lymphatic function, lymphangiogenesis and cancer metastasis. Microscopy Res. 2001; 55:92-99. Review. Article Link

Skobe M, Detmar M. Structure, function, and molecular control of the skin lymphatic system. J. Invest. Dermatol. Symp. Proc. 2000; 14-19. Article Link

Book Chapters

Rogic A, Auger F, Skobe M. (2018) Isolation of human skin lymphatic endothelial cells and 3D reconstruction of the lymphatic vasculature in vitro. In: Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Oliver G and Kahn M (Eds.), Humana press, New York, NY pp. 279-290. Book Chapter Link

Fusenig NE, Skobe M, Vosseler S, Hansen M, Lederle W, Airola K, Tomakidi P, Stark H-J, Boukamp P, Breitkreutz D. (2002) Tissue models to study tumor stroma interactions, In: Proteases and their inhibitors in cancer metastasis. Foidart J-M and Muschel RJ (Eds.), Kluwer Academic Publishers, Netherlands, pp. 205-223. Book Chapter Link