Karki Laboratory

Brain, Vagus and Body Mechanisms of Fear and Stress

Research

Extreme stress and/or fear can profoundly affect brain and body mechanisms leading to increased vulnerability for developing disorders such as post-traumatic stress (PTSD) and panic disorders. Stress exerts its effects on the brain and body via the sympathetic and parasympathetic systems which allow organisms to tackle a variety of stressors with “fight or flight” responses regulated by the sympathetic nervous system (SNS), and “rest and digest” responses, regulated by the parasympathetic nervous system (PNS).  Extreme or prolonged activation of the sympathetic systems can have detrimental effects at the level of neurons and astrocytes in various brain regions, neurotransmitter and neuropeptidergic systems, and organs like heart, lungs and gut in turn affecting central, metabolic, cardiorespiratory and immune functions. The vagus nerve, a major cranial nerve connecting the brain and body and known as the “wandering nerve”, is an important regulator of parasympathetic functions via innervations to the thoracic and abdominal organs, and the autonomic, cardiovascular, respiratory, gastrointestinal, immune, and endocrine systems. Direct vagus nerve stimulation using electrical devices or through mind/body practices such as yoga appear to be effective in diminishing stress, immune, and cardiorespiratory functions.

Karki lab is dedicated to understanding the reciprocal mechanisms between the brain and body axis via the sympathetic and parasympathetic modulators that affect or are affected by traumatic stress and associated changes in cardiorespiratory functions. Our research questions span along three main areas: 1) How is the brain-vagus-body axis involved in modulating fear and stress? 2) What are the mechanisms that link traumatic stress and metabolic syndromes?, and, 3) How are brain astrocytes involved in regulating fear, stress and cardiorespiratory functions? Our work is focused on the neuropeptidergic systems like PACAP (pituitary adenylate cyclase activating peptide) and CRF (corticotropin releasing factor) and the neurotransmitter norepinephrine. To answer our questions, we use cutting-edge tools and techniques such as optogenetics, chemogenetics, genetically modified mouse lines, intersectional viral approach, telemetry tracking of cardiorespiratory functions, behavioral models of traumatic stress and fear, and many others.

Contact Us

Karki Laboratory
Abha Karki Rajbhandari
Principal Investigator
abha.rajbhandari@mssm.edu

Current Projects

1. How is the brain-vagus-body axis involved in modulating fear and stress?

This projects seeks to investigate the role of reciprocal connections between the brain and body (heart and lungs) via the vagus nerve in regulating traumatic fear, stress and cardiorespiratory functions.

2. What are the mechanisms that link traumatic stress and metabolic syndromes?

This project investigates the link between traumatic stress and metabolic changes with relevance to obesity, diabetes and other metabolic syndromes in stress.

3. How are brain astrocytes involved in regulating fear and stress?

This project is investigating the role of brain astrocytes and their modulation by neuropeptides in regulation of fear, stress and cardiorespiratory functions.

Funding & Awards

Current Funding support:

2020   Brain and Behavior Research Foundation NARSAD Young Investigator Grant

2020   Whitehall Foundation Grant

2020   Akira Arimura Young Investigator Grant for VIP/PACAP Research

2020   Friedman Brain Institute Scholars Award

Honors:

2019   Arimura Award for Young Investigators for Excellence in VIP/PACAP Research

2018   Brain and Behavior Research Foundation NARSAD Young Investigator Award

2015   Ruth L. Kirschstein National Research Service Award (NRSA) Individual Postdoctoral Fellowship

2015   Trainee Professional Development Award, Society of Neuroscience

2015   Brain Research Institute Travel Award for Society of Neuroscience, UCLA

2012   Vilas Conference Grant for Society for Neuroscience Conference

2011   Competitive Travel Award (Neuroscience Training Program)

2010   Competitive Training Grant-T32 GM007507

2006   Social Science award, Olivet College

2004-2006: President’s List, Olivet College

2004-2006: Dean’s List, Olivet College

2004-2005: Heritage grant, Olivet College

2004-2005: S-Varney Scholarship, Olivet College

Recent Publications

Rajbhandari A, Octeau CJ, Gonzalez S, Pennington ZT, Trott J, Chavez J, Ngyuen E, Keces N, Hong W, Neve RL, Waschek J, Khakh BS, Fanselow MS. A peptidergic amygdala microcircuit modulates sexually dimorphic contextual fear. bioRxiv. 2020;

Team

Abha Karki Rajbhandari
Principal Investigator
abha.rajbhandari@mssm.edu

Farzanna Mohamed
Research Associate
farzanna.mohamed@mssm.edu

Shade Eleazer
Research Assistant
seleazer@mmm.edu

Kennedy Blankenship
kblankenship@mmm.edu

Alumni

Sydney Hart, Research Associate. Current-Graduate Student at NYU
Nazia Fyazi, Research Associate

Positions Available

Contact Us

Abha Karki Rajbhandari
Principal Investigator
abha.rajbhandari@mssm.edu