KEY PUBLICATIONS

2022-2023

ArifT., Qiu J., Menon, A., MenonV., LinM., Benson D.L., TzavarasN., and S. Ghaffari. Repression of hyperactivated lysosomes restores youthful properties in old hematopoietic stem cells (In revision).

Kalair A., Pavan M., Alpert N., Ghaffari S., and E. Taioli. Blood inflammatory markers and mortality in the US population: A Health and Retirement Survey (HRS) analysis. PLOS ONE, Oct 16, 2023.

Liang R.*, Lin M.*, Menon V., Qiu, J., Menon A., Breda L., Arif T., Rivella S., and S. Ghaffari. Elevated CDKN1a (p21) mediates β-thalassemia erythroid apoptosis but its loss does not improve β-thalassemic erythropoiesis. Blood Advances, Sep 6, 2023.

S. Ghaffari. Hematopoietic stem cell quiescence exposed using mitochondrial membrane potential. Current Opinion in Hematology, Jan 1, 2023.

Qiu J. and S. Ghaffari. Mitochondrial deep dive into hematopoietic stem cell dormancy: not much glycolysis but plenty of sluggish lysosomes. Experimental Hematology online, July 29, 2022.

Qiu J., Menon V., Tzavaras N., Liang R., and S. Ghaffari. A protocol for identifying and analyzing mouse and human quiescent hematopoietic stem cells using flow cytometry and confocal imaging. STAR Protocols, Dec 16, 2022.

2020 – 2021

Qiu J., Gjini J., Arif T., Moore K., Lin M., and S. Ghaffari. Using mitochondrial activity to select for potent human hematopoietic stem cells. Blood Adv., Mar 23, 2021.

Liang R., Menon V., Qiu J., Arif T., Renuse S., Lin M., Nowak R., Hartmann B., Tzavaras N., Benson D., Chipuk J., Fribourg M., Pandey A., Fowler V., and S. Ghaffari. Mitochondrial localization and moderated activity are key to murine erythroid enucleation. Blood Adv., May 25, 2021.

Menon V. and S. Ghaffari. Erythroid enucleation: A gateway into a “bloody” world. Experimental Hematology, Mar, 2021.

Tremblay D., Rapp J., Alpert N., Lieberman-Cribbin W., Mascarenhas J., Taioli E., and S. Ghaffari. Mild anemia as a single independent predictor of mortality in patients with COVID-19. eJHaem, May 6, 2021.

Rapp J., Tremblay D., Alpert N., Lieberman-Cribbin W., Mascarenhas J., Taioli E., and S. Ghaffari. Red cell distribution width is associated with mortality in non-anemic patients with COVID-19. Journal of Medical Virology, Apr 10, 2021.

Liang R.^*, Arif T.^*, Kalmykova S., Kasianov A., Lin M., Menon V., Qiu J., Bernitz J., Moore K., Lin F., Benson D., Tzavaras N., Mahajan M., Papatsenko D., and S. Ghaffari. Restraining lysosomal activity Preserves Hematopoietic Stem Cell Quiescence and Potency. Cell Stem Cell, Mar 5, 2020.

Ghaffari S. Lysosomal Regulation of Metabolism in Quiescent Hematopoietic Stem Cells: More Than Just Autophagy. Cell Stem Cell, Mar 4, 2021.

2019

Filippi MD. and S. Ghaffari. Mitochondria in the maintenance of hematopoietic stem cells: new perspectives and opportunities. Blood, May 2, 2019.

Liang R., Menon V., and S. Ghaffari. Following Transcriptome to Uncover FOXO Biological Functions. Methods in Molecular Biology, 2019.

2018

Liu J., Duan Z., Guo W., Zeng L., Wu Y., Chen Y., Tai F., Wang Y., Lin Y., Zhang Q., He Y., Deng J., Stewart R., Wang C., Lin P., Ghaffari S., Evers B., Liu S., Zhou M., Zhou B., and J. Shi. Targeting the BRD4/FOXO3a/CDK6 axis sensitizes AKT inhibition in luminal breast cancer. Nature Communication, Dec 5, 2018.

Liang R. and S. Ghaffari. Stem Cells Seen Through the FOXO Lens: An Evolving Paradigm. Current Topics in Developmental Biology, 2018.

Menon V. and S. Ghaffari. Transcription factors FOXO in the regulation of homeostatic hematopoiesis. Current Opinions in Hematology, July 2018.

Liang R*., Camprecios G*., Kou Y., McGrath K., Nowak R., Catherman S., Bigarella C.L., Rimmelé P., Zhang X., Gnanapragasam M.N., Bieker J.J., Papatsenko D., Ma’yan A., Bresnick E., Fowler V., Palis J., and S. Ghaffari. A systems approach identifies essential FOXO3’s functions at key steps of terminal erythropoiesis. PLoS Genetics, Oct 9, 2015.

Price J., Idoyaga J., Salmon H., Hogstad B., Bigarella C., Ghaffari S., Leboeuf M., and M. Merad. CDKN1A regulates Langerhans cell survival and promotes Treg cell generation upon exposure to ionizing irradiation. Nature Immunology, Oct 2015.

2014

Rimmelé P., Bigarella C.L., Liang R., Izac B., Dieguez-Gonzalez R., Barbet G., Donovan M., Brugnara C., Blander J.M., Sinclair D.A., and S. Ghaffari. Aging-like phenotype and defective lineage specification in SIRT1-deleted hematopoietic stem and progenitor cells. Stem Cell Reports, 2014.

In the news: Protein that keeps blood stem cells healthy as they age identified by researchers. Science Daily, June 9, 2014.

S. Ghaffari. PIAS adds methyl-bias to HSC-differentiation. EMBO Journal. 2014.

Rimmelé P., Lofek S., and S. Ghaffari. Resveratrol increases the bone marrow hematopoietic stem and progenitor cell capacity. American Journal of Hematology, 2014.

Bigarella, C.*, Liang R.*, and S. Ghaffari. Stem cells and the impact of ROS signaling. Development, 2014.

Zhang X., Camprecios G., Yalcin S., Mungamuri S.H., Barminko J., Rimmele P., Liang R., Baron M., Brugnara C., Papatsenko D., Rivella S., and S. Ghaffari. FOXO3-mTOR metabolic cooperation in the regulation of erythroid cell maturation and homeostasis. American Journal of Hematology, 2014.

McIver S.C., Kang Y.A., DeVilbiss A.W., O’Driscoll C.A., Ouellette J.N., Pope N.J., Camprecios G., Chang C-J, Yang D., Bouhassira E.E., S. Ghaffari, and E.H. Bresnick. The Exosome complex establishes a barricade to erythroid maturation. Blood, 2014.

Liang R. and S. Ghaffari. Stem cells, redox signaling, and stem cell aging. Antioxidant and Redox Signaling, Apr 20, 2014.

2011 – 2013

Ramos P., Casu C., Gardenghi S., Breda L., Crielaard B., Guy E., Marongiu M.F., Gupta R., Levine R.L., Abdel-Wahab O., Ebert B.L., Van Rooijen N., Ghaffari S., Grady R.W., Giardina P.J., and S. Rivella. Macrophages support pathological erythropoiesis in polycythemia vera and -thalassemia. Nature Medicine, Mar 17, 2013.

Zhang X., Yalcin S., Lee D.F., Lee S-M, Yeh T.Y.J., Jie S., Kennedy M., Sellers R., Landthaler, M., Tuschl T, Chi N.W., Lemischka I., Keller G., and S. Ghaffari. FOXO1 is an essential regulator of pluripotency in human embryonic stem cells. Nature Cell Biology, July 21, 2011.

Zhang X., Rielland M., Yalcin S., and S. Ghaffari. Regulation and function of FoxO transcription factors in normal and cancer stem cells: what have we learned? Current Drug Targets, Aug 1, 2011.

S. Ghaffari. Cancer, Stem Cells and Cancer Stem Cells: Old Ideas, New Developments. F1000 Medicine Reports, Dec 1, 2011.

Rimmelé P., Zhang X., and S. Ghaffari. FoxO proteins in the control of stem cells. Revue Médecine Sciences. 2012.

2006 – 2010

Yalcin S., Marinkovic D., Mungamuri S.K., Zhang X., Tong W., and S. Ghaffari. ROS-mediated amplification of AKT/mTOR signalling pathway leads to myeloproliferative syndrome in Foxo3(-/-) mice. EMBO Journal, Nov 26, 2010.

Yu D., dos Santos C.O., Zhao G., Jiang J., Amigo J.D., Khandros E., Dore L.C., Yao Y., D’Souza J., Zhang Z., Ghaffari S., Choi J., Friend S., Tong W., Orange J.S., Paw B.H., and M.J. Weiss. miR-451 protects against erythroid oxidant stress by repressing 14-3-3z. Genes and Development, 2010.

Yalcin S., Zhang X., Marinkovic D., Luciano J.P., Sarkar A., Brugnara C., Vercherat C., Taneja R., and S. Ghaffari. Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells. Journal of Biological Chemistry, 2008.

Marinkovic D., ZhangX., Yalcin S., Brugnara C., Huber T., and S. Ghaffari. Foxo3 is required for the regulation of oxidative stress in erythropoiesis. Journal of Clinical Investigation, 2007.

Sun H., Ghaffari S., and R. Taneja. bHLH-OrangeTranscription Factors in Development and Cancer. Translational Oncogenomics, 2007.

S. Ghaffari. Oxidative stress in the regulation of normal and neoplastic hematopoiesis. (Forum Issue). Antioxidants & Redox Signaling, 2008.

Jagani Z., Song K., Kutok J.L., Dewar M.R., Melet A., Santos T., Grassian A., Ghaffari S., Wu C., Ren R., Rodin H.Y., Miller K., Khosravi-Far R. Proteasome inhibition causes regression of leukemia and abrogates BCR-ABL-induced evasion of apoptosis in part through regulation of forkhead tumor suppressors. Cancer Research, 2009.

Vercherat C., Chung T.K., Yalcin S., Gulbagci N., Gopinadhan S., Ghaffari S., and R. Taneja. Stra13 regulates oxidative stress mediated skeletal muscle degeneration. Human Molecular Genetics, 2009.

Pervaiz S., Taneja R., and S. Ghaffari. Oxidative Stress Regulation of Stem and Progenitor Cells. (Forum Issue). Antioxidants & Redox Signaling, 2009.

2003 – 2005

Zhao W., Kitidis C., Fleming M.D., Lodish H.F., and S. Ghaffari. Erythropoietin stimulates phosphorylation and activation of GATA-1 via the PI3-kinase/AKT signaling pathway. Blood, Feb 1, 2006.

Ghaffari S., Kitidis C., Zhao W., Marinkovic D., Fleming M.D., Luo B., Marszalek J., and H F. Lodish. AKT induces erythroid-cell maturation of JAK2-deficient fetal liver progenitor cells and is required for Epo regulation of erythroid-cell differentiation. Blood, Mar 1, 2006.

Ghaffari S., Jagani Z., Kitidis C., Lodish H.F., and R. Khosravi-Far. Cytokines and BCR-ABL mediate suppression of TRAIL-induced apoptosis through inhibition of forkhead FOXO3a transcription factor. Proceedings of the National Academy of Science, May 27, 2003.