[et_pb_section fb_built=”1″ fullwidth=”on” admin_label=”Page Header” _builder_version=”4.9.0″ background_image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/01\/business-12.jpg” parallax=”on” animation_direction=”top”][et_pb_fullwidth_header title=”Research Initiatives” text_orientation=”center” admin_label=”Hero Section” _builder_version=”4.9.0″ title_font=”Montserrat|on|||” title_font_size=”60px” title_line_height=”1.4em” content_font_size=”22px” content_line_height=”1.8em” subhead_font=”||||” background_color=”rgba(255, 255, 255, 0)” use_background_color_gradient=”on” background_color_gradient_start=”rgba(58,52,226,0.93)” background_color_gradient_end=”rgba(73,108,220,0.7)” background_image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/03\/20220806_20012311008-scaled.jpg” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_button_one=”on” button_one_text_size=”16px” button_one_bg_color=”#6eba01″ button_one_border_width=”2px” button_one_border_color=”#6eba01″ button_one_letter_spacing=”2″ button_one_font=”Montserrat|||on|” button_one_use_icon=”off” custom_button_two=”on” button_two_text_size=”16px” button_two_border_width=”2px” button_two_border_color=”#ffffff” button_two_letter_spacing=”2″ button_two_font=”Montserrat|||on|” button_two_use_icon=”off” module_alignment=”left” animation_style=”fade” button_one_text_color_hover=”#6eba01″ button_two_text_color_hover=”#6eba01″ button_one_border_color_hover=”#ffffff” button_two_border_color_hover=”#ffffff” button_one_border_radius_hover=”0″ button_two_border_radius_hover=”0″ button_one_letter_spacing_hover=”2″ button_two_letter_spacing_hover=”2″ button_one_bg_color_hover=”#ffffff” button_two_bg_color_hover=”#ffffff” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_one_text_color__hover_enabled=”on” button_one_text_color__hover=”#6eba01″ button_two_text_color__hover_enabled=”on” button_two_text_color__hover=”#6eba01″ button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_one_border_color__hover_enabled=”on” button_one_border_color__hover=”#ffffff” button_two_border_color__hover_enabled=”on” button_two_border_color__hover=”#ffffff” button_one_border_radius__hover_enabled=”on” button_one_border_radius__hover=”0″ button_two_border_radius__hover_enabled=”on” button_two_border_radius__hover=”0″ button_one_letter_spacing__hover_enabled=”on” button_one_letter_spacing__hover=”2″ button_two_letter_spacing__hover_enabled=”on” button_two_letter_spacing__hover=”2″ button_one_bg_color__hover_enabled=”on” button_one_bg_color__hover=”#ffffff” button_two_bg_color__hover_enabled=”on” button_two_bg_color__hover=”#ffffff”]<\/p>\n
Check out below the current research topics that we are exploring.<\/p>\n
<\/p>\n
[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_column type=”1_2″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_blurb title=”Viral-Host Interactions” image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/02\/D8-20X-PL-FL-Phase_GFP-469525DAPI-377447_1_001-smaller.jpeg” _builder_version=”4.9.0″ _module_preset=”default” transform_origin=”256%|160%”]<\/p>\n
During a viral infection, viruses <\/span>can<\/span> hijack the host machinery <\/span>to <\/span>enhance <\/span>their <\/span>replication and<\/span> counteract host defenses.<\/span> By identifying the host factors that the virus utilize<\/span>s<\/span> we can develop or repurpose drugs to negate the virus-host interaction, allowing for the cell to better defend and impede viral replication. In our analysis of SARS-CoV-2 virus-host interactions <\/span>we<\/span> i<\/span>n cooperation with the Nevan <\/a><\/span>Krogan<\/span><\/a> Lab<\/a>, have identified 332 host factors important for its infection<\/span>. With these findings we were able to identify host-targeted <\/span>plitidepsin<\/span>, already <\/span>clinically-approved<\/span> treatment for multiple myeloma, as a SARS-CoV-2 therapeutic. The White lab continues to explore host-virus interactions in SARS-CoV-2 and Influenza virus to better develop and identify <\/span>new <\/span>therapeutic<\/span> treatments.<\/span><\/span>\u00a0<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_blurb title=”Cell Culture & In-Vivo Models” image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/01\/20230117_130951-smaller.jpg” _builder_version=”4.9.0″ _module_preset=”default” locked=”off”]<\/p>\n The White lab <\/span>has a dedicated Tecan D300e and <\/span>Biomek<\/span> i7 to automate our cell culture systems and screen antiviral drugs in high throughput<\/span> formats<\/span>. <\/span>Cell culture models are critical<\/span> in the development of antiviral therapeutics. Screening of antiviral <\/span>drugs in standard cell lines that can be infected <\/span>by the target virus<\/span> is a ubiquitous early stage of drug development<\/span>. We <\/span>also <\/span>use these cell lines for <\/span>initial screening, but <\/span>these cell lines are plagued with genomic abnormalities that reduce their relevance to<\/span> the human host. Therefore, we employ primary<\/span> (HAE on ALI)<\/span> and primary-like <\/span>(iPSC) <\/span>models for advanced analysis of antiviral leads to <\/span>quantify the efficacy of the compound accurately.<\/span> <\/span>We have also established animal models <\/span>of viral infection for the analysis of antiviral drugs for coronaviruses, flaviviruses and enteroviruses. Therefore, the White lab <\/span>encompasses<\/span> <\/span>all<\/span> the<\/span> critical preclinical analysis <\/span>required <\/span>to determine antiviral efficacy of <\/span>therapeutic candidates.<\/span><\/span>\u00a0<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.9.0″ _module_preset=”default” custom_padding=”0px|||||”][et_pb_column type=”1_2″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_blurb title=”Drug Combination Studies” image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/02\/D8-40X-PL-FL-Phase_GFP-469525DAPI-377447_1_001-smaller.jpeg” _builder_version=”4.9.0″ _module_preset=”default”]<\/p>\n Antiviral drug<\/span> combination therapies have been <\/span>remarkably <\/span>effective<\/span> in the clinic<\/span> against<\/span> <\/span>HIV (H<\/span>uman<\/span> <\/span>Immunodeficiency<\/span> V<\/span>irus<\/span>)<\/span> and <\/span>HCV (hepatitis C virus)<\/span>. <\/span>These <\/span><\/span>chronic viral infections<\/span> necessitate the use of drug combination<\/span>s to prevent the inevitable emergence of resistance from monotherapy antiviral strategies.<\/span> Acute viral infections, like those established by<\/span> many<\/span> respiratory viruses, <\/span>also<\/span> are<\/span> at high risk of the development of antiviral resistance. This is due to the high mutation rate of RNA viruses, which <\/span>leads to the constant development of viral variants.<\/span> <\/span><\/span>Furthermore<\/span>, the use of a single drug in the treatment of an infection could necessitate the use of a high dos<\/span>age<\/span> <\/span>resulting<\/span> in<\/span> <\/span>increase<\/span>d<\/span> <\/span>toxicity<\/span>.<\/span> <\/span>The use of two drugs targeting different viral <\/span>proteins <\/span>allows for each drug in the combination to inhibit the replication <\/span>of viruses resistant to the partner drug.<\/span> <\/span>T<\/span>he White lab continues to explore the mechanism<\/span>s<\/span> behind synergistic drug interactions and assess potential clinical candidates that can take advantage of this antiviral synergy to improve potency and prevent the development of resistance in the clinic for Coronavirus, Flavivirus and Enterovirus<\/span><\/span>.\u00a0<\/span><\/span>\u00a0<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.9.0″ _module_preset=”default”][et_pb_blurb title=”Antiviral Resistance Analysis” image=”https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-content\/uploads\/sites\/433\/2023\/01\/IMG-0559-rotated.jpg” _builder_version=”4.9.0″ _module_preset=”default”]<\/p>\n Antiviral resistance is <\/span>a prominent concept in the development of antiviral drugs. This is because the mutational rate <\/span>of viruses is orders of magnitude larger than other pathogens such as bacteria and parasites.<\/span> Therefore, the potential for an antiviral drug to generate resistance mutations<\/span> in a viral population is <\/span>a critical step in the development of novel therapeutics. The <\/span>W<\/span>hite lab uses automated NGS<\/span> library preparation and analysis to track antiviral resistance mutations under compound selective<\/span> pressure in our cell culture and animal models.<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Check out below the current research topics that we are exploring. During a viral infection, viruses can hijack the host machinery to enhance their replication and counteract host defenses. By identifying the host factors that the virus utilizes we can develop or repurpose drugs to negate the virus-host interaction, allowing for the cell to better […]<\/p>\n","protected":false},"author":576,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-81","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/pages\/81","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/users\/576"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/comments?post=81"}],"version-history":[{"count":12,"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/pages\/81\/revisions"}],"predecessor-version":[{"id":292,"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/pages\/81\/revisions\/292"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/whitelab\/wp-json\/wp\/v2\/media?parent=81"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}