{"id":115,"date":"2015-02-05T19:18:11","date_gmt":"2015-02-05T19:18:11","guid":{"rendered":"http:\/\/labs.icahn.mssm.edu\/parsonslab\/?page_id=115"},"modified":"2021-03-09T20:27:47","modified_gmt":"2021-03-09T20:27:47","slug":"115-2","status":"publish","type":"page","link":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/projects\/115-2\/","title":{"rendered":"Chromatin"},"content":{"rendered":"<h2 style=\"text-align: center\"><\/h2>\n<h6><a href=\"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-content\/uploads\/sites\/93\/2015\/02\/David_24.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-126 alignnone\" src=\"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-content\/uploads\/sites\/93\/2015\/02\/David_24.jpg\" alt=\"\" width=\"1952\" height=\"2209\" srcset=\"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-content\/uploads\/sites\/93\/2015\/02\/David_24.jpg 1952w, https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-content\/uploads\/sites\/93\/2015\/02\/David_24-265x300.jpg 265w, https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-content\/uploads\/sites\/93\/2015\/02\/David_24-905x1024.jpg 905w\" sizes=\"auto, (max-width: 1952px) 100vw, 1952px\" \/><\/a><\/h6>\n<h3 style=\"text-align: justify\"><\/h3>\n<h2 style=\"text-align: center\">Chromatin Regulation and Tumor Suppression<\/h2>\n<p style=\"text-align: justify\">Mammalian SWI\/SNF (mSWI\/SNF) complexes are multi-subunit chromatin remodeling complexes that alter the connection between DNA and histone proteins.\u00a0 mSWI\/SNF activity can subsequently result in transciptional activation or repression, depending on the gene and cellular context.\u00a0 All mSWI\/SNF complexes contain one of two mutually exclusive ATP-dependent DNA-helicases (either BRM or BRG1).\u00a0 They also contain accessory subunits that are thought to confer functional specificity\u00a0<em>in vivo<\/em>, by altering where the complexes bind in the genome and what co-factors they bind.\u00a0 BAF180, a 180-kDa protein encoded by the PBRM1 gene<em>,\u00a0<\/em>is one such accessory subunit: it is one of the defining subunits of the PBAF mSWI\/SNF complex. \u00a0We found that PBRMI\/BAF180 is mutated in breast cancer and regulates activation of P21. \u00a0A multitude of recent sequencing studies have revealed that mSWI\/SNF components are widely mutated in cancer.\u00a0 For example, PBRM1 mutations are found in ~40% of clear cell renal cell carcinoma (ccRCC) patients, making it the second most highly mutated gene in ccRCC (behind VHL).\u00a0 In this project, we are investigating the mechanisms by which BAF180 functions as a tumor suppressor.<\/p>\n<p style=\"text-align: justify\">We are also highly interested in the role that PTEN plays in the regulation of gene expression and chromatin access, since we have recently shown that PTEN interacts with RNA polymerase II and its transcription regulation machinery.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chromatin Regulation and Tumor Suppression Mammalian SWI\/SNF (mSWI\/SNF) complexes are multi-subunit chromatin remodeling complexes that alter the connection between DNA and histone proteins.\u00a0 mSWI\/SNF activity can subsequently result in transciptional activation or repression, depending on the gene and cellular context.\u00a0 All mSWI\/SNF complexes contain one of two mutually exclusive ATP-dependent DNA-helicases (either BRM or BRG1).\u00a0 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":91,"menu_order":4,"comment_status":"open","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-115","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/pages\/115","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/comments?post=115"}],"version-history":[{"count":3,"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/pages\/115\/revisions"}],"predecessor-version":[{"id":440,"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/pages\/115\/revisions\/440"}],"up":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/pages\/91"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/parsonslab\/wp-json\/wp\/v2\/media?parent=115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}