{"id":14,"date":"2016-06-07T21:01:26","date_gmt":"2016-06-07T21:01:26","guid":{"rendered":"http:\/\/labs.icahn.mssm.edu\/panlab\/?page_id=14"},"modified":"2016-06-07T21:24:57","modified_gmt":"2016-06-07T21:24:57","slug":"ubiquitin-ligases","status":"publish","type":"page","link":"https:\/\/labs.icahn.mssm.edu\/panlab\/ubiquitin-ligases\/","title":{"rendered":""},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15\" src=\"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-content\/uploads\/sites\/90\/2016\/06\/Ubiquitin.png\" alt=\"Ubiquitin\" width=\"900\" height=\"535\" srcset=\"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-content\/uploads\/sites\/90\/2016\/06\/Ubiquitin.png 900w, https:\/\/labs.icahn.mssm.edu\/panlab\/wp-content\/uploads\/sites\/90\/2016\/06\/Ubiquitin-300x178.png 300w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/p>\n<p><strong>The mechanism of ubiquitination by the Cullin-RING E3 ubiquitin ligases<\/strong><\/p>\n<p style=\"text-align: justify\">Cullin-RING E3 ubiquitin ligases (CRLs) are the largest RING-type of E3 family consisting of \u2248240 members, nearly half of E3s identified in humans. CRL is responsible for ~20% of protein degradation mediated by the 26S proteasome. CRL dys-regulation contributes directly to human diseases including cancer. Canonical CRLs are modular complexes, in which a cullin\u2019s N terminal domain assembles interchangeably with different CUL-specific substrate receptors capable of binding a substrate. On the other hand, the C-terminal half of a cullin (cullin CTD) binds a RING finger protein, ROC1\/RBX1 for CUL1-CUL4 or ROC2\/RBX2 for CUL5, to form a core ligase complex. CRL\u2019s core ligase can collaborate with specific E2 conjugating enzymes to catalyze the transfer of Ub to the bound substrate and the subsequent polyubiquitination reaction. A major focus of our lab is to explore mechanistic insights into the polyubiquitination reaction orchestrated by CRL.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The mechanism of ubiquitination by the Cullin-RING E3 ubiquitin ligases Cullin-RING E3 ubiquitin ligases (CRLs) are the largest RING-type of E3 family consisting of \u2248240 members, nearly half of E3s identified in humans. CRL is responsible for ~20% of protein degradation mediated by the 26S proteasome. CRL dys-regulation contributes directly to human diseases including cancer. [&hellip;]<\/p>\n","protected":false},"author":146,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-14","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/pages\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/users\/146"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":2,"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"predecessor-version":[{"id":53,"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/pages\/14\/revisions\/53"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/panlab\/wp-json\/wp\/v2\/media?parent=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}