{"id":40,"date":"2016-07-11T18:06:15","date_gmt":"2016-07-11T18:06:15","guid":{"rendered":"http:\/\/labs.icahn.mssm.edu\/krulwichlab\/?page_id=40"},"modified":"2016-08-17T18:59:31","modified_gmt":"2016-08-17T18:59:31","slug":"publications","status":"publish","type":"page","link":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/publications\/","title":{"rendered":"Publications"},"content":{"rendered":"

2015-2000<\/p>\n

Preiss, L., D.B. Hicks, S. Suzuki, T. Meier, and T.A. Krulwich. 2015. Alkaliphilic bacteria with impact on industrial applications, concepts of early life forms, and bioenergetics of ATP synthesis. Front. Bioeng. Biotechnol. 3:75. Doi: 10.3389\/bioe.s015.00075. PMCID:PMC4453477.<\/p>\n

DeCaen, P.G., Y. Takahashi, T.A. Krulwich, M. Ito, and D.E. Clapham. 2014. Ionic selectivity and thermal adaptations within the voltage-gated sodium channel family of alkaliphilic Bacillus<\/em>. Elife doi: 10.7554\/eLife.04387. PMID:PMC4225499.<\/p>\n

Attie, O., A. Jayaprakash, H. Shah, I.T. Paulsen, M. Morino, Y. Takahashi, I. Narummi, R. Sachidanandam, K. Satoh, M. Ito, and T.A. Krulwich. 2014. Draft genome sequence of Bacillus alcalophilus<\/em> AV1934, a classic alkaliphile isolated from human feces in 1934. Genome Announc. 13:2. E01175-14, doi: 10.1128\/genomeA.01175-14.<\/p>\n

Preiss, L., J.D. Langer, D.B. Hicks, J. Liu, \u00d6. Yildiz, T.A. Krulwich and T. Meier. 2014. The structure of the c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus <\/em>OF4 is adapted to the alkaliphilic lifestyle. Molec. Microbiol. 92:973-984. PMCID:PMC4064006<\/p>\n

Liu, J., S. Ryabichko, M. Bogdanov, O.J. Fackelmayer, W. Dowhan and T.A. Krulwich. 2014. Cardiolipin is dispensable for oxidative phosphorylation and non-fermentative growth of alkaliphilic Bacillus pseudofirmus <\/em>OF4. J. Biol. Chem. 289:2960-2971. PMC3908427<\/p>\n

Morino, M., T. Suzuki, M. Ito and T.A. Krulwich. 2014. Purification and functional reconstitution of a multi-subunit Mrp type Na+<\/sup>\/H+<\/sup> antiporter. <\/em>J. Bacteriol. 196:28-35 PMC3911140<\/p>\n

Price-Whelan, A., C.K. Poon, M.A. Benson, C.M. Roux, P.M. Dunman, V.J. Torres, and T.A. Krulwich. 2013. Transcriptional profiling of Staphylococcus aureus<\/em> during growth in 2 M NaCl leads to clarification of physiological roles for Kdp and Ktr K+<\/sup> uptake systems. mBio, vol 4 4e00407-13 PMC3747578<\/p>\n

Krulwich, T. A. and M. Ito. 2013. Prokaryotic alkaliphiles. In: The Prokaryotes, Vol. 1, 4th ed. E. Rosenberg, E.F. DeLong, F. Thompson, S. Lory, E. Stackebrandt (eds) Springer New York, invited review.<\/p>\n

Preiss, L., A.L. Klyszejko, D.B. Hicks, J. Liu, O. Fackelmayer, T.A. Krulwich, and T. Meier. 2013. The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus<\/em> OF4. Proc. Natl. Acad. Sci. USA. PMC3651500<\/p>\n

Liu, J., D.B. Hicks, and T.A. Krulwich. 2013. Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus<\/em> OF4 in ATP synthase assembly and non-fermentative growth. J. Bacteriol. PMC3553844.<\/p>\n

Krulwich, T.A., G. Sachs, and E. Padan. 2011. Molecular aspects of bacterial pH sensing and homeostasis. Nature Microbiol. Rev. 9: 330-343. PMC3247762<\/p>\n

Janto, B., A. Ahmed, M. Ito, J. Liu, D.B. Hicks, S. Pagni, O.J. Fackelmayer, T.A. Smith, J. Earl, L.D.H. Elbourne, K. Hassan, I.T. Paulsen, A.-B. Kolst\u00f8, N.J. Tourasse, G.D. Ehrlich, R. Boissy, D.M. Ivey, G. Li, Y. Xue, Y. Ma, F.Z. Hu and T.A. Krulwich. 2011. The genome of alkaliphilic Bacillus pseudofirmus <\/em>OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4. Environ. Microbiol. 13: 3289-3309. PMC3228905<\/p>\n

Liu, J., O. Fackelmayer, D. B. Hicks, L. Preiss, T. Meier, E. Sobie and T. A. Krulwich. 2011. Mutations in a helix-1 motif of the ATP synthase c-<\/em>subunit of Bacillus<\/em> pseudofirmus <\/em>OF4 cause functional deficits and changes in stability and mobility of the c<\/em>-rotor on SDS-PAGE. <\/em>Biochemistry 50: 5496-5506. <\/em>PMC3115392<\/p>\n

Krulwich, T. A., J. Liu, M. Morino, M. Fujisawa, M. Ito, and D. B. Hicks. 2010. Adaptive mechanisms of extreme alkaliphiles. In: Extremophiles Handbook. Horikoshi, K., Antranikian, G., Bull, A., Robb, F., Stetter, K. (eds), Springer, Heidelberg.<\/em><\/p>\n

Fujisawa, M., O. Fackelmayer, J. Liu, D. B. Hicks and T. A. Krulwich. 2010. The ATP Synthase a-<\/em>subunit of extreme alkaliphiles is a distinct variant. J. Biol. Chem. 285: 32105-32115.<\/em> PMC2952212<\/p>\n

Morino, M., S. Natsui, T. Ono, T. H. Swartz, T. A. Krulwich and M. Ito. 2010. Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus <\/em>OF4. J. Biol. Chem. 285: 30942-30950. <\/em>PMC2945585<\/p>\n

Hoffmann, J., L. Sokolova, L. Preiss, D.B. Hicks, T.A. Krulwich, N. Morgner, T. Meier, I. Wittig, H. Sch\u00e4gger and B. Brutschy. 2010. ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry. Phys. Chem. Chem. Phys. 12: 13375-13382.<\/em> PMC2955850<\/p>\n

Preiss, L., \u00d6. Yildiz, D.B. Hicks, T.A. Krulwich and T. Meier. 2010. A new type of proton coordination in an F1<\/sub>F0<\/sub>-ATP synthase rotor ring. PLoS Biol. 8:e1000443.<\/em> PMC2914638<\/p>\n

Slonczewski, J. L., M. Fujisawa, M. Dopson and T. A. Krulwich. 2009. Cytoplasmic pH measurement and homeostasis in bacteria and archaea. Adv. Microbial Physiol. 55:1-79.<\/p>\n

Liu, J., M. Fujisawa, D. B. Hicks and T. A. Krulwich. 2009. Characterization of the functionally critical AXAXAXA and PXXEXXP motifs of the ATP synthase c<\/em>-subunit from an alkaliphilic Bacillus. <\/em>J. Biol. Chem. 284: 8714\u20138725.<\/p>\n

Fujinami, S, N. Terahara, T. A. Krulwich and M. Ito. 2009. Motility and chemotaxis in alkaliphilic Bacillus <\/em>species. Future Microbiol. 4: 1137-1149.<\/p>\n

Terahara, N., T. A. Krulwich and M. Ito. 2009. Mutations alter the sodium versus proton use of a Bacillus clausii <\/em>flagellar motor and confer dual ion use on Bacillus subtilis <\/em>motors. Proc. Natl. Acad. Sci. USA 105:14359-14364.<\/p>\n

Dzioba-Winogrodzki, J., O. Winogrodzki, T. A. Krulwich, M. A. Boin, C. C. Hase and P. Dibrov. 2009. The Vibrio cholerae<\/em> Mrp system: cation\/proton antiport properties and enhancement of bile salt-resistance in a heterologous host. J. Mol. Microbiol. Biotech 16: 176-186.<\/p>\n

Morino, M., S. Natsui, T.H. Swartz, T.A. Krulwich and M. Ito. 2008. Single gene deletions of mrpA <\/em>to mrpG <\/em>and mrpE <\/em>point mutations affect activity of the Mrp Na+<\/sup>\/H+<\/sup> antiporter of alkaliphilc Bacillus <\/em>and formation of hetero-oligomeric Mrp complexes. J. Bacteriol. 190: 4162-4172.<\/p>\n

\u00a0<\/em>Krulwich, T. A., D. B. Hicks, T. Swartz and M. Ito. 2007. Bioenergetic adaptations that support alkaliphily. In<\/em>: Physiology and Biochemistry of Extremophiles (C. Gerday and N. Glansdorff, eds.), 311-329, ASM Press, Washington, DC<\/p>\n

Fujinami, S., T. Sato, J.S. Trimmer, B.W. Spiller, D.E. Clapham, T.A. Krulwich, I. Kawagishi and M. Ito. 2007. The voltage-gated Na+<\/sup> channel NaV<\/sub>BP co-localizes with methyl-accepting chemotaxis proteins at cell poles of alkaliphilic Bacillus pseudofirmus<\/em> OF4. Microbiology 153: 4027-4038].<\/em><\/p>\n

Fujisawa, M., M. Ito and T. A. Krulwich. 2007. Three 2-component transporters with channel-like properties have monovalent cation\/proton antiport activity. Proc. Natl. Acad. Sci. USA 104: 13289-13294.<\/em><\/p>\n

Wei, Y., J. Liu, Y. Ma and T. A. Krulwich. 2007. Three putative cation\/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica <\/em>N10 complement an alkali-sensitive Escherichia coli <\/em>mutant. Microbiology 153: 2168-2179.<\/em><\/p>\n

Swartz, T. H., M. Ito, T. Ohira, S. Natsui, D. B. Hicks and T. A. Krulwich. 2007. Catalytic properties of Staphylococcus aureus <\/em>and Bacillus<\/em> members of the secondary Cation-Proton Antiporter-3 family are revealed by an optimized assay in an Escherichia coli <\/em>host. J. Bacteriol. 189: 3081-3090.<\/p>\n

Liu, X., X. Gong, D. B. Hicks, T. A. Krulwich, L. Yu and C.-A. Yu. 2007. Interaction between the cytochrome caa3<\/sub><\/em> and F1<\/sub>F0<\/sub>-ATP synthase of alkaliphilic Bacillus pseudofirmus <\/em>OF4 is demonstrated by saturation transfer electron paramagnetic resonance and differential scanning calorimetry assays. Biochemistry 46: 306-313.<\/em><\/p>\n

Wei, Y., G. Deikus, B. Powers, V. Shelden, T. A. Krulwich and D. H. Bechhofer. 2006. Adaptive gene expression in Bacillus subtilis <\/em>strains deleted for tetL<\/em>. J. Bacteriol. 188: 7090-7100.<\/em><\/p>\n

Terahara, N., M. Fujisawa, B. Powers, T. M Henkin, T. A. Krulwich and M. Ito. 2006. An intergenic stem-loop mutation in the Bacillus subtilis ccpA-motPS <\/em>operon increases motPS <\/em>transcription and the contribution of MotPS to motility. J. Bacteriol. 188: 2701-2705.<\/p>\n

Padan, E., E. Bibi, M. Ito and T. A. Krulwich. 2005. Alkaline pH homeostasis in bacteria: new insights. Biochim. Biophys. Acta 1717: 67-88.<\/em><\/p>\n

Swartz, T. H., S. Idewada, O. Ishikawa, M. Ito and T. A. Krulwich. 2005. The Mrp system: a giant among monovalent cation\/proton antiporters? Extremophiles 9:345-354.<\/p>\n

Krulwich, T. A., O. Lewinson, E. Padan and E. Bibi. 2005. Do physiological roles foster persistence of drug\/multidrug-efflux pumps? A case study. Nature Rev. Microbiol. 3:566-572.<\/em><\/p>\n

Liu, J., Y. Xue, Q. Wang, Y. Wei, T. Swartz, D. B. Hicks, Y. Ma and T. A. Krulwich. 2005. The activity profile of the NhaD-type Na+<\/sup>(Li+<\/sup>)\/H+<\/sup> antiporter from the soda lake alkaliphile Alkalimonas amylolytica <\/em>is adaptive for its extreme environment. J. Bacteriol. 187: 7589-7595.<\/p>\n

Ito, M., N. Terahara, S. Fujinami and T. A. Krulwich. 2005. Properties of motility in Bacillus subtilis<\/em> powered by the H+<\/sup>-coupled MotAB flagellar stator, Na+<\/sup>-coupled MotPS or hybrid stators MotAS and MotPB. J. Mol. Biol. 352:396-408.<\/em><\/p>\n

De Jesus, M., J. Jin, A. A. Guffanti and T. A. Krulwich. 2005. Importance of the GP dipeptide of the antiporter motif and other membrane-embedded proline and glycine residues in tetracycline efflux protein Tet(L). Biochemistry 44:12896-12904.<\/em><\/p>\n

Swartz, T. H., M. Ito, D. B. Hicks, M. Nuqui, A. A. Guffanti and T. A. Krulwich. 2005. The Mrp Na+<\/sup>\/H+<\/sup> antiporter increases activity of the malate:quinone oxidoreductase of an Escherichia coli <\/em>respiratory mutant. J. Bacteriol. 187:388-391.<\/p>\n

Rivera-Torres, I. O., R. D. Krueger-Koplin, D. B. Hicks, S. M. Cahill, T. A. Krulwich and M. E. Girvin. 2004. pKa<\/sub> <\/em>of the essential Glu54 and backbone conformation for subunit c<\/em> from the H+<\/sup>-coupled F1<\/sub>F0 <\/sub>ATP synthase from an alkaliphilic Bacillus<\/em>. FEBS Lett. 575:131-135.<\/p>\n

Ito, M., H. Xu, A. A. Guffanti, Y. Wei, L. Zvi, D. E. Clapham and T. A. Krulwich. 2004. The voltage-gated Na+<\/sup> channel NaV<\/sub>BP has a role in motility, chemotaxis and pH homeostasis of an alkaliphilic Bacillus<\/em>. Proc. Natl. Acad. Sci. U. S. A. 101:10566-10571 .<\/p>\n

Ito, M., D. B. Hicks, T. M. Henkin, A. A. Guffanti, B. Powers, K. Uematsu and T. A. Krulwich. 2004. MotPS is the stator-force generator for motility of alkaliphilic Bacillus<\/em> and its homologue is a second functional Mot in Bacillus subtilis<\/em>. Mol. Microbiol. 53:1035-1049.<\/p>\n

Wang, Z., D. B. Hicks, A. A. Guffanti, K. Baldwin, and T. A. Krulwich. 2004. Replacement of amino acid sequence features of a-<\/em> and c-<\/em>subunits of ATP synthases of alkaliphilic Bacillus<\/em> with the Bacillus<\/em> consensus sequence results in defective oxidative phosphorylation and non-fermentative growth at pH 10.5. J. Biol. Chem. 279:26446-26554.<\/p>\n

Kreuger-Koplin, R. D., P. L. Sorgen, S. T. Krueger-Koplin, I. O. Rivera-Torres, S.M. Cahill, D. B. Hicks, L. Grinius, T. A. Krulwich, and M. E. Girvin. 2004. An evaluation of detergents for NMR structural studies of membrane proteins. J. Biomol. NMR 17: 43-57.<\/p>\n

Safferling, M., H. Griffith, J. Jin, J. Sharp, M. De Jesus, C. Ng, T. A. Krulwich and D.N. Wang. 2003. TetL tetracycline efflux protein from Bacillus subtilis<\/em> is a dimer in the membrane and in detergent solution. Biochemistry 42:13969-13976.<\/p>\n

Hicks, D. B., Z. Wang, Y. Wei, R. Kent, A. A. Guffanti, H. Banciu, D. H. Bechhofer and T. A. Krulwich. 2003. A newly discovered tenth atp<\/em> gene and the conserved atpI<\/em> gene of a Bacillus atp<\/em> operon have a role in Mg2+<\/sup> uptake. Proc. Natl. Acad. Sci. U.S.A. 100:10213-10218.<\/p>\n

Wei, Y., T.W. Southworth, H. Kloster, M. Ito, A.A. Guffanti, A. Moir, and T.A. Krulwich. 2003. Mutational loss of a K+<\/sup> and NH4<\/sub>+<\/sup> transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus<\/em> OF4. J. Bacteriol. 185:5133-5147.<\/p>\n

Guffanti, A.A., Y. Wei, S.V. Rood and T.A. Krulwich. 2002. An antiport mechanism for a member of the Cation Diffusion Facilitator Family: divalent cations efflux in exchange for K+<\/sup> and H+<\/sup>. Mol. Microbiol. 45:145-153.<\/p>\n

Jin, J., A.A. Guffanti, D.H. Bechhofer and T.A. Krulwich. 2002. Tet(L) and Tet(K) tetracycline-divalent metal\/H+<\/sup> antiporters: characterization of multiple catalytic modes and a mutagenesis approach to difference in their efflux substrate and coupling ion preferences. J. Bacteriol. 184:4722-4732.<\/p>\n

Southworth, T.W., A.A. Guffanti, A. Moir and T.A. Krulwich. 2001. GerN: an endospore germination protein of Bacillus cereus<\/em> is a Na+<\/sup>\/H+<\/sup>-K+<\/sup> antiporter. J. Bacteriol. <\/em>183:5896-5903.<\/p>\n

Ito, M., A.A. Guffanti, and T.A. Krulwich. 2001. Mrp-dependent Na+<\/sup>\/H+<\/sup> antiporters of Bacillus<\/em> exhibit characteristics that are unanticipated for completely secondary active transporters. FEBS Lett. 496:117-120.<\/em><\/p>\n

Jin, J., A.A. Guffanti, C. Beck, and T.A. Krulwich. 2001. A 12-transmembrane segment (TMS) version (\u2206TMS VII-VIII) of the 14-TMS Tet(L) antibiotic-resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity. J. Bacteriol. 183:2667-2671.<\/p>\n

Gilmour, R, P. Messner, A.A. Guffanti, R. Kent, A. Scheberl, N. Kendrick, and T.A. Krulwich. 2000. Two-dimensional gel electrophoresis analyses of pH-dependent protein expression in facultatively alkaliphilic Bacillus pseudofirmus<\/em> OF4 lead to characterization of an S-layer protein with a role in alkaliphily. J. Bacteriol. 182:5969-5981.<\/p>\n

Ito, M., A.A. Guffanti, W. Wang, and T.A. Krulwich. 2000. Effects of non-polar mutations in each of the seven Bacillus subtilis<\/em> mrp<\/em> genes suggest complex interactions among the gene products in support of Na+<\/sup>– and alkali- but not cholate-resistance. J. Bacteriol. 182:5663-5670.<\/p>\n

Wei, Y., A.A. Guffanti, M. Ito, and T.A. Krulwich. 2000. Bacillus subtilis<\/em> YqkI is a novel Malic\/NaLactate antiporter that enhances growth on malate at low protonmotive force. J. Biol. Chem. 275:30287-30292.<\/p>\n

Wang.W., A.A. Guffanti, Y. Wei, M. Ito and T.A. Krulwich. 2000. Two types of Bacillus subtilis tetA<\/em>(L) deletion strains reveal the physiological importance of TetA(L) in K+<\/sup> acquisition as well as in Na+<\/sup>-, alkali-, and tetracycline resistance. J. Bacteriol. 182:2088-2095.<\/p>\n

Takami, H. and T.A. Krulwich. 2000. Re-identification of facultatively alkaliphilic Bacillus firmus<\/em> OF4 as Bacillus pseudofirmus<\/em> OF4. Extremophiles 4:19-22.<\/p>\n

1999-1990<\/p>\n

Ito, M., A.A. Guffanti, B. Oudega, and T.A. Krulwich. 1999. mrp<\/em>: a multigene, multifunctional locus in Bacillus subtilis<\/em> with roles in resistance to cholate and to Na+<\/sup>, and in pH homeostasis. J. Bacteriol. 181:2394-2402.<\/p>\n

Wei, Y., A.A. Guffanti and T.A. Krulwich. 1999. Sequence analysis and functional studies of a chromosomal region of alkaliphilic Bacillus firmus<\/em> OF4 encoding an ABC-type transporter with similarity of sequence and Na+ <\/sup>exclusion capacity to the Bacillus subtilis<\/em> NatAB transporter. Extremophiles 3:113-118.<\/p>\n

Gronstad, A., E. Jaroszewicz, M. Ito, M.G. Sturr, T.A. Krulwich, and A.B. Kolsto. 1998. Physical map of alkaliphilic Bacillus firmus<\/em> OF4 and detection of a large endogenous plasmid. Extremophiles 2:447-453.<\/p>\n

Ito, M., A.A. Guffanti, J. Zemsky, D.M. Ivey, and T.A. Krulwich. 1997. The role of the nhaC<\/em>-encoded Na+<\/sup>\/H+<\/sup> antiporter of alkaliphilic Bacillus firmus<\/em> OF4. J. Bacteriol. 179:3851-3857.<\/p>\n

Cheng, J., A.A. Guffanti, and T.A. Krulwich. 1997. A two gene ABC-type transport system involved in Na+<\/sup> extrusion by Bacillus subtilis<\/em> is induced by ethanol and protonophore. Mol. Microbiol. 23:1107-1120.<\/p>\n

Gilmour, R., and T.A. Krulwich. 1997. Construction and characterization of a mutant of alkaliphilic Bacillus firmus<\/em> OF4 with a disrupted cta<\/em> operon and purification of a novel cytochrome bd<\/em>. J. Bacteriol. 179:863-870.<\/p>\n

Ito, M., B. Cooperberg, and T.A. Krulwich. 1997. Diverse genes of alkaliphilic Bacillus firmus<\/em> OF4 that complement K+<\/sup> uptake-deficient Escherichia coli<\/em> include an ftsH<\/em> homologue. Extremophiles 1:22-28.<\/p>\n

Gilmour, R., and T.A. Krulwich. 1996. Purification and characterization of the succinate dehydrogenase complex and CO-reactive b<\/em>-type cytochromes from the facultative alkaliphile Bacillus firmus<\/em> OF4. Biochim. Biophys. Acta 1276:57-63.<\/p>\n

Cheng, J., A.A. Guffanti, W. Wang, T.A. Krulwich, and D.H. Bechhofer. 1996. Chromosomal tetA<\/em>(L) gene of Bacillus subtilis<\/em>: regulation of expression and physiology of a tetA<\/em>(L) deletion strain. J. Bacteriol. 178:2853-2860.<\/p>\n

Cheng, J., K. Baldwin, A.A. Guffanti, and T.A. Krulwich. 1996. The Na+<\/sup>\/H+<\/sup> antiport activity conferred by the Bacillus subtilis tetA<\/em>(L) gene, a 5′ truncation product of tetA<\/em>(L), and related plasmid genes upon Escherichia coli<\/em>. Antimicrob. Agents Chemother. 40:852-857.<\/em><\/p>\n

Sturr, M.G., T.A. Krulwich, and D.B. Hicks. 1996. Purification of a cytochrome bd<\/em> terminal oxidase encoded by the Escherichia coli app<\/em> locus from a \u0394cyo\u0394cyd<\/em> strain complemented by genes from Bacillus firmus<\/em> OF4. J. Bacteriol. 176:1742-1749.<\/p>\n

Krulwich, T.A. 1995. Alkaliphiles: “basic” molecular problems of pH tolerance and bioenergetics. Molec. Microbiol. 15:403-410.<\/p>\n

Guffanti, A.A. and T.A. Krulwich. 1995. Tetracycline\/H+<\/sup> antiport and Na+<\/sup>\/H+<\/sup> antiport catalyzed by the Bacillus subtilis<\/em> Tet(L) transporter expressed in Escherichia coli<\/em>. J. Bacteriol. 177:4557-4561.<\/p>\n

Cheng, J., A.A. Guffanti, and T.A. Krulwich. 1994. The chromosomal tetracycline-resistance locus of Bacillus subtilis<\/em> encodes a Na+<\/sup>\/H+<\/sup> antiporter that is physiologically important at elevated growth pH. J. Biol. Chem. 269:27365-27371<\/p>\n

Guffanti, A.A. and T.A. Krulwich. 1994. Oxidative phosphorylation by ADP + Pi-loaded membrane vesicles from alkaliphilic Bacillus firmus<\/em> OF4. J. Biol. Chem. 269:21576-21582.<\/p>\n

Ivey, D.M., M.G. Sturr, T.A. Krulwich, and D.B. Hicks. 1994. The abundance of atp <\/em>gene transcript and of the mem\u00adbrane F1<\/sub>Fo<\/sub>-ATPase as a function of the growth pH of alkaliphilic Bacil\u00adlus firmus<\/em> OF4. J. Bacteriol. 176:5167-5170.<\/p>\n

Hicks, D.B., D. Cohen, and T.A. Krulwich. 1994. Reconstitu\u00adtion of the energy-linked activities of the solubilized F1<\/sub>F0<\/sub> ATP synthase from Ba\u00adcillus subtilis<\/em>. J. Bacteriol. 176:4192-4195.<\/p>\n

Sturr, M.G., A.A. Guffanti, and T.A. Krulwich. 1994. Growth and bioenergetics of alkaliphilic Bacillus firmus<\/em> OF4 in continuous culture at high pH. J. Bacteriol. 176:3111-3116.<\/p>\n

Quirk, P.G., A.A. Guffanti, S. Clejan, J. Cheng, and T.A. Krulwich. 1994. Isolation of Tn917<\/em> insertional mutants of Bacillus subtilis<\/em> that are resistant to the protonophore carbonyl cyanide m<\/em>-chlorophenylhydrazone. Biochim. Biophys. Acta 1186:27-34.<\/p>\n

Ivey, D.M., A.A. Guffanti, J. Zemsky, E. Pinner, R. Karpel, S. Schuldiner, E. Padan, and T.A. Krulwich. 1993. Cloning and character\u00adization of a putative Ca+2<\/sup>\/H+<\/sup> antiporter gene from Escherichia coli<\/em> upon func\u00adtional complementation of Na+<\/sup>\/H+<\/sup> antiporter-deficient strains by the overexpressed gene. J. Biol. Chem. 268:11296-11303.<\/p>\n

Quirk, P.G., E.A. Dunkley, Jr., P. Lee, and T.A. Krulwich. 1993. Identification of a putative Bacillus subtilis rho<\/em> gene. J. Bacteriol. 175:647-654.<\/p>\n

Quirk, P.G., D.B. Hicks, and T.A. Krulwich. 1993. Cloning of the cta<\/em> operon from alkaliphilic Bacillus firmus<\/em> OF4, and characteriza\u00adtion of the pH-regulated cy\u00adto\u00adchrome caa<\/em>3<\/sub> oxidase it encodes. J. Biol. Chem. 268:678-685.<\/p>\n

Ivey, D.M., J. Cheng, and T.A. Krulwich. 1992. A 1.6 kb region of Bacillus firmus<\/em> OF4 DNA encodes a homolog of Escherichia coli<\/em> and yeast DNA topoisomerases and may con\u00adtain a translational read-through of UGA. Nucleic Acids Res. 20:4928.<\/p>\n

Khan, S., D.M. Ivey, and T.A. Krulwich. 1992. Mem\u00adbrane ultrastructure of alkaliphilic Bacillus<\/em> species stud\u00adied by rapid freeze electron microscopy. J. Bacteriol. 174:5123-5126.<\/p>\n

Ivey, D.M., A.A. Guffanti, Z.H. Shen, N. Kudyan, and T.A. Krulwich. 1992. The cad<\/em>C gene product of alkaliphilic Bacillus firmus<\/em> OF4 partially restores Na+<\/sup>-resistance to an Escherichia coli<\/em> strain lack\u00ading an Na+<\/sup>\/H+<\/sup> antiporter (NhaA). J. Bacteriol. 174:4878-4884.<\/p>\n

Moody, A.J., R.B. Gennis, D.B., Hicks, W.J.., Ingledew, W.J., T.A. Krulwich, J.C. Rumbley, and P.R. Rich. 1992. Ligand binding properties of bacterial oxidases in rela\u00adtion to cytochrome c<\/em> oxidase. Biochem. Soc. Trans. 20:240S.<\/p>\n

Guffanti, A.A. and T.A. Krulwich. 1992. Features of apparent non-chemiosmotic energization of oxidative phos\u00adphorylation by alkaliphilic Bacillus firmus<\/em> OF4. J. Biol. Chem. 267:9580-9588.<\/p>\n

Ivey, D.M. and T.A. Krulwich. 1992. Two unrelated alkaliphilic Bacillus<\/em> species possess identical de\u00advi\u00ada\u00adtions in sequence from those of conventional prokaryotes in re\u00adgions of Fo<\/sub> genes implicated in proton transloca\u00adtion through the ATP synthase. Res. Microbiol. 143:467-470.<\/p>\n

Ivey, D.M., A.A. Guffanti, J.S. Bossewitch, E. Padan, and T.A. Krulwich. 1991. Molecular cloning and sequenc\u00ading of a gene from alkaliphilic Bacillus firmus<\/em> OF4 that func\u00adtional\u00adly complements an Escherichia coli<\/em> strain carrying a dele\u00adtion in the nha<\/em>A Na+<\/sup>\/H+<\/sup> antiporter gene. J. Biol. Chem. 266:23483-23489.<\/p>\n

Ivey, D.M. and T.A. Krulwich. 1991. Structure and nu\u00adcleotide sequence of the genes encoding the ATP syn\u00adthase from alkaliphilic Bacillus firmus<\/em> OF4. Mol. Gen. Genet. 229:292-300.<\/p>\n

Quirk, P., A.A. Guffanti, R.J. Plass, S. Clejan, and T.A. Krulwich. 1991. Protonophore-resistance and cyto\u00adchrome expression in mutant strains of the faculta\u00adtive alkaliphile Bacillus firmus<\/em> OF4. Biochim. Biophys. Acta 1058:131-140.<\/p>\n

Ivey, D.M., D.B. Hicks, A.A. Guffanti, G. Sobel, and T.A. Krulwich. 1990. The problem of the electrochemical proton potential in alkaliphilic bacteria. Mosbach Collo\u00adquium (Springer-Verlag, Heidelberg) 41:105-113.<\/p>\n

Krulwich, T.A., P.G. Quirk, and A.A. Guffanti. 1990. Uncoupler-resistant mutants of bacteria. Microbiol. Revs. 54:52-65.<\/p>\n

Krulwich, T.A. and D.M. Ivey. 1990. Bioenergetics in extreme environments. In<\/em>: The Bacteria: A Treatise on Structure and Function, J. Sokatch and N. Ornston, eds., Volume 12, Bacterial Energetics, T. Krulwich, ed., Aca\u00addem\u00adic Press, Orlando, Florida.<\/p>\n

Hicks, D.B. and T.A. Krulwich. 1990. Purification and reconstitution of the F1<\/sub>Fo<\/sub>-ATP synthase from alkaliphilic Bacillus firmus<\/em> OF4: evidence that the enzyme translocates H+<\/sup> but not Na+<\/sup>. J. Biol. Chem. 265:20547-20554.<\/p>\n

Ivey, D.M. and T.A. Krulwich. 1990. Sequence of the gene encoding the ATP synthase beta subunit from alkaliphilic Bacillus firmus<\/em> RAB. Nucl. Acids Res. 18:1296.<\/p>\n

1989-1979<\/p>\n

Krulwich, T.A. and A.A. Guffanti. 1989. Alkalophilic bacteria. Ann. Rev. Microbiol. 43:435-463.<\/p>\n

Rohde, M., F. Mayer, D.B. Hicks, and T.A. Krulwich. 1989. Immunoelectron microscopic localization of the F1<\/sub>Fo<\/sub>ATPase (ATP synthase) on the cytoplasmic mem\u00adbrane of alkalophilic Bacillus firmus<\/em> RAB. Biochim. Biophys. Acta 985:233-235.<\/p>\n

Clejan, S., A.A. Guffanti, M.A. Cohen, and T.A. Krulwich. 1989. Mutation of Bacillus firmus<\/em> OF4 to duramycin resistance results in substan\u00adtial replacement of membrane lipid phosphatidylethanolamine by its plasmal\u00adogen form. J. Bacteriol. 171:1744-1746.<\/p>\n

Clejan, S., and T.A. Krulwich. 1988. Permeability studies of lipid vesicles from alkalophilic Bacillus firmus<\/em> showing opposing effects of mem\u00adbrane iso\u00adpren\u00adoid and diacylglycerol fractions and suggest\u00ading a pos\u00adsible basis for obligate alkalophily. Biochim. Biophys. Acta 946: 40-48.<\/p>\n

Guffanti, A.A. and Krulwich, T.A. 1988. ATP synthesis is driven by an imposed \u0394pH or \u0394\u03bcH+<\/sub> but not by an im\u00adposed \u0394pNa+<\/sup> or \u0394\u03bcNa+<\/sub> in alkalophilic Bacillus firmus<\/em> OF4 at high pH. J. Biol. Chem. 263:14748-14752.<\/p>\n

Dunkley, E.A., Jr., S. Clejan, A.A. Guffanti, and T.A. Krulwich. 1988. Large decreases in membrane phosphatidyl\u00adethanolamine and diphosphatidylglycerol upon mutation to duramycin resistance do not change the protonophore resis\u00adtance of Bacillus subtilis<\/em>. Biochim. Biophys. Acta 943:13-18.<\/p>\n

Davidson, M.W., K.A. Gray, D.B. Knaff, and T.A. Krulwich. 1988. Purification and characterization of two soluble cytochromes from the alkalophile Bacillus firmus<\/em> RAB. Biochim. Biophys. Acta 933:470-477.<\/p>\n

Clejan, S., A.A. Guffanti, L.H. Falk, and T.A. Krulwich. 1988. The protonophore resistance of Bacillus megaterium<\/em> is correlated with elevated ra\u00adtios of sat\u00adu\u00adrat\u00aded to unsatu\u00adrated fatty acids in membrane phospholipids. Biochim. Biophys. Acta 932:43-51.<\/p>\n

Hicks, D.B. and T.A. Krulwich. 1987. Studies of the F1<\/sub> ATPase from Bacillus subtilis<\/em> and its uncoupler-resistant mutant derivatives. J. Bacteriol. 169:4743-4749.<\/p>\n

Krulwich, T.A., S. Clejan, L. Falk, and A.A. Guffanti. 1987. Incorporation of specific exoge\u00adnous fatty acids into membrane lipids modulates protonophore resistance in Ba\u00adcillus subtilis<\/em>. J. Bacteriol. 169:4479-4485.<\/p>\n

Guffanti, A.A., S. Clejan, L.H. Falk, D.B. Hicks, and T.A. Krulwich. 1987. Isolation and characterization of uncoupler-resistant mutants of Bacillus subtilis<\/em>. J. Bacteriol. 169:4469-4478.<\/p>\n

Clejan, S., T.A. Krulwich, K.R. Mondrus, and D. Seto-Young. 1986. Membrane lipid composition of obligately and facultatively alkalophilic strains of Bacillus<\/em>. J. Bacteriol. 168:334-340.<\/p>\n

Hicks, D.B. and T.A. Krulwich. 1986. The membrane ATP\u00adase of alkalophilic Bacillus firmus<\/em> RAB is an F1<\/sub>-type ATPase. J. Biol. Chem. 261:12896-12902.<\/p>\n

Guffanti, A.A., O. Finkelthal, D.B. Hicks, L. Falk, A. Sidhu, A. Garro, and T.A. Krulwich. 1986. Isolation and characterization of new facultatively alkalophilic strains of Bacillus<\/em>. J. Bacteriol. 167:766-773.<\/p>\n

Krulwich, T.A., A.A. Guffanti, M.Y. Fong, L. Falk, and D.B. Hicks. 1986. Alkalophilic Bacillus firmus<\/em> RAB gen\u00ader\u00adates variants which can grow at lower Na+<\/sup> concentra\u00adtions than the parental strain. J. Bacteriol. 165:884-889.<\/p>\n

Seto-Young, D., M.L. Garcia, and T.A. Krulwich. 1985. Reconstitution of a bacterial Na+<\/sup>\/H+<\/sup> antiporter. J. Biol. Chem. 260:11393-11395.<\/p>\n

Krulwich, T.A., R. Agus, M. Schneier, and A.A. Guffanti. 1985. The buffering capacity of bacilli that grow in different ranges of pH. J. Bacteriol. 162:768-772.<\/p>\n

Krulwich, T.A., J.G. Federbush, and A.A. Guffanti. 1985. Presence of a non-metabolizable solute that is translocated with Na+<\/sup> enhances Na+<\/sup>-dependent pH homeosta\u00adsis in an alkalophilic Bacillus<\/em>. J. Biol. Chem. 260:4055-4058.<\/p>\n

Guffanti, A.A., E. Chiu, and T.A. Krulwich. 1985. Failure of an alkalophilic bacterium to synthe\u00adsize ATP in re\u00adsponse to a valinomycin-induced potassium diffu\u00adsion po\u00adtential at high pH. Arch. Biochem. Biophys. (is\u00adsue hon\u00ador\u00ading B.L. Horecker) 239:327-333.<\/p>\n

Guffanti, A.A., M. Mann, T.L. Sherman, and T.A. Krulwich. 1984. Patterns of electrochemical proton gradi\u00adent formation by membrane vesi\u00adcles from an obligately aci\u00addo\u00adphilic bacterium. J. Bacteriol. 159:448-452.<\/p>\n

Kitada, M. and T.A. Krulwich. 1984. Purification and characterization of the cytochrome oxidase from alkalophilic Bacillus firmus<\/em> RAB. J. Bacteriol. 158:963-967.<\/p>\n

Guffanti, A.A., R.T. Fuchs, M. Schneier, E. Chiu, and T.A. Krulwich. 1984. A \u0394\u03a8-generated by respiration is not equivalent to a diffusion potential of the same magni\u00adtude for ATP synthesis by Bacillus firmus<\/em> RAB. J. Biol. Chem. 259: 2971-2975.<\/p>\n

Garcia, M.L., A.A. Guffanti, and T.A. Krulwich. 1983. Characterization of the Na+<\/sup>\/H+ <\/sup>antiporter of alkalophilic bacilli in vivo<\/em>: \u0394\u03a8-dependent 22<\/sup>Na+<\/sup> efflux from starved cells. J. Bacteriol. 156:1151-1157.<\/p>\n

Krulwich, T.A. 1983. Na+<\/sup>\/H+<\/sup> antiporters. Biochim. Biophys. Acta 726:245-264.<\/p>\n

Kitada, M., A.A. Guffanti, and T.A. Krulwich. 1982. Bioenergetic properties and viability of the alkalophilic Bacillus firmus<\/em> RAB as a function of pH and Na+<\/sup> content of the medium. J. Bacteriol. 152:1096-1104.<\/p>\n

Bonner, S., M. Mann, A.A. Guffanti, and T.A. Krulwich. 1982. Na+<\/sup>\/solute symport in membrane vesicles from Bacil\u00adlus alcalophilus<\/em>. Biochim. Biophys. Acta 679:315-322.<\/p>\n

Lewis, R.J., R. Prince, P.L. Dutton, D. Knaff, and T.A. Krulwich. 1981. The respiratory chain of Bacillus alcalophilus<\/em> and its non-alkalophilic mu\u00adtant de\u00adriv\u00ada\u00adtive. J. Biol. Chem. 256:10543-10549.<\/p>\n

Guffanti, A.A., H. Blumenfeld, and T.A. Krulwich. 1981. ATP synthesis by an uncoupler_resistant mutant of Bacil\u00adlus megaterium<\/em>. J. Biol. Chem. 256:8416-8421.<\/p>\n

Guffanti, A.A., R.F. Bornstein, and T.A. Krulwich. 1981. Oxidative phosphorylation by membrane vesicles from Bacillus alcalophilus<\/em>. Biochim. Biophys. Acta 635:619-630.<\/p>\n

Mandel, K.G., A.A. Guffanti, and T.A. Krulwich. 1980. Monovalent cation\/proton antiporters in membrane vesi\u00adcles from Bacillus alcalophilus<\/em>. J. Biol. Chem. 225:7391-7396.<\/p>\n

Krulwich, T.A., K.G. Mandel, R.F. Bornstein, and A.A. Guffanti, 1979. A non-alkalophilic mutant of Bacillus alcalophilus <\/em>lacks the Na+<\/sup>\/H+<\/sup> antiporter. Biochem. Biophys. Res. Comm. 91: 58-62.<\/p>\n

Krulwich, T.A. and N. Pelliccione. 1979. Catabolic pathways of coryneforms, nocardia and mycobacteria. Ann. Rev. Microbiol. 33:95-111.<\/p>\n

Pelliccione, N., B. Jaffin, M.E. Sobel and T.A. Krulwich. 1979. Induction of the phosphoenolpyruvate:hexose phosphotransferase system associated with relative anaerobiosis in an obligate aerobe. Eur. J. Biochem. 95: 69-75.<\/p>\n

Note: <\/strong>Apart from the research efforts detailed above Terry Krulwich has been engaged in outreach efforts to enhance diversity in academic science and elsewhere; currently specific efforts include Program Directorship of Mount Sinai PREP (Post-baccalaureate Research Education Program; which is sponsored by NIGMS and in its 15th<\/sup> year). Several publications related to diversity efforts are cited below.<\/p>\n

Butts, G.C., Y. Hurd, A.G. Palermo, A.G., D. Delbrune, S. Saran, C. Zony, and T.A. Krulwich. 2012. Role of institutional climate in fostering diversity in biomedical research workforce: a case study. Mt. Sinai J. Med. 79:498-511.<\/p>\n

McGee, R., Jr., Saran, S., and T.A. Krulwich. 2012. Diversity in the biomedical research workforce: developing talent. Mt. Sinai J. Med. 79: 397-411.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

2015-2000 Preiss, L., D.B. Hicks, S. Suzuki, T. Meier, and T.A. Krulwich. 2015. Alkaliphilic bacteria with impact on industrial applications, concepts of early life forms, and bioenergetics of ATP synthesis. Front. Bioeng. Biotechnol. 3:75. Doi: 10.3389\/bioe.s015.00075. PMCID:PMC4453477. DeCaen, P.G., Y. Takahashi, T.A. Krulwich, M. Ito, and D.E. Clapham. 2014. Ionic selectivity and thermal adaptations within […]<\/p>\n","protected":false},"author":146,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width.php","meta":{"footnotes":""},"class_list":["post-40","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/pages\/40","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/users\/146"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/comments?post=40"}],"version-history":[{"count":5,"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/pages\/40\/revisions"}],"predecessor-version":[{"id":57,"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/pages\/40\/revisions\/57"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/krulwichlab\/wp-json\/wp\/v2\/media?parent=40"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}