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horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; text_line_height=&#8221;1.8em&#8221;]<\/p>\n<p><strong>Our Research<\/strong><\/p>\n<p>[\/et_pb_text][et_pb_text admin_label=&#8221;Research&#8221; _builder_version=&#8221;4.9.0&#8243; text_font=&#8221;Open Sans||||||||&#8221; text_text_color=&#8221;#000000&#8243; text_font_size=&#8221;18px&#8221; text_text_shadow_horizontal_length=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; text_text_shadow_vertical_length=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_vertical_length_tablet=&#8221;0px&#8221; text_text_shadow_blur_strength=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_blur_strength_tablet=&#8221;1px&#8221; link_text_shadow_horizontal_length=&#8221;link_text_shadow_style,%91object Object%93&#8243; link_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; link_text_shadow_vertical_length=&#8221;link_text_shadow_style,%91object Object%93&#8243; 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header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; text_line_height=&#8221;1.8em&#8221;]<\/p>\n<p>The Kostic Lab focuses on drug discovery and development in autism spectrum disorder (ASD). The lab\u2019s main goals are to identify potential drug candidates for treatment of ASD by designing experimental strategies for testing in neuronal cell systems and animal models, as well as to discover and validate molecular biomarkers in ASD. Specifically, the lab focuses on ADNP, DDX3X, FOXP1, and Phelan-McDermid syndromes, common single-gene causes of autism.<\/p>\n<p>&nbsp;<\/p>\n<p>The Kostic Lab leads the Drug Discovery and Development (DDD) program at the <a href=\"https:\/\/icahn.mssm.edu\/research\/seaver\">Seaver Autism Center for Research and Treatment\u00a0<\/a>at the\u00a0<a href=\"https:\/\/icahn.mssm.edu\/\">Icahn School of Medicine at Mount Sinai<\/a>. The Seaver Autism Center is a fully integrated, translational research center that leads progressive studies and provides personalized care to individuals with autism and related rare disorders. As a team, we are dedicated to discovering the biological causes of autism and developing breakthrough treatments. \u00a0\u00a0<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/icahn.mssm.edu\/research\/seaver\/research\">Learn more<\/a>\u00a0about the Seaver Autism Center\u2019s translational research.<\/p>\n<p>[\/et_pb_text][et_pb_divider _builder_version=&#8221;4.9.0&#8243; divider_position=&#8221;center&#8221; divider_weight=&#8221;0.5px&#8221; module_alignment=&#8221;center&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; prev_background_color=&#8221;#000000&#8243; next_background_color=&#8221;#000000&#8243; _builder_version=&#8221;4.9.0&#8243; background_color=&#8221;#ffffff&#8221; 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Seaver Foundation<\/p>\n<p>ADNP Kids Research Foundation<\/p>\n<p>Seaver Foundation Fellowship<\/p>\n<p>[\/et_pb_text][et_pb_divider _builder_version=&#8221;4.9.0&#8243; divider_position=&#8221;center&#8221; divider_weight=&#8221;0.5px&#8221; module_alignment=&#8221;center&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; prev_background_color=&#8221;#000000&#8243; next_background_color=&#8221;#000000&#8243; _builder_version=&#8221;4.9.0&#8243; background_color=&#8221;#ffffff&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; 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header_4_text_shadow_blur_strength=&#8221;header_4_text_shadow_style,%91object Object%93&#8243; header_4_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_5_text_shadow_horizontal_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_5_text_shadow_vertical_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_5_text_shadow_blur_strength=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<p><strong><em>Development of high-throughput in vitro assays for drug screening and validation<\/em><\/strong><\/p>\n<p>The DDD program is adopting an approach of utilizing inducible pluripotent stem cells (iPSCs) to develop cellular assays for <em>in vitro <\/em>drug candidate screening and validation. Currently, our in-house approach focuses on characterization of (1) neuronal morphological features and synaptic puncta, along with (2) neuronal network activity in human neurons generated from iPSC lines (patient-derived and in CRISP\/Cas9 engineered) harboring ADNP, SHANK3, DDX3X and FOXP1 mutations and their respective control lines with the final aim of developing an in vitro human model for ASD that can be used as a platform for drug screening and validation.<\/p>\n<p>Neuronal morphology and synaptic puncta characterization of induced neurons (iN) modeling monogenic forms of ASD \u2013 Our aim is to describe for the first time an in-depth high-content phenotypic characterization in human ASD <em>in vitro<\/em> models by establishing and optimizing a protocol for the characterization of neuronal morphology features and synaptic density. The methodology involves large-scale generation of mature neurons followed by an automated and simultaneous measurement of neuronal shape and synaptic density using the High-Content Screening (HCS) Platforms.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-51 aligncenter\" src=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-1.png\" alt=\"\" width=\"799\" height=\"333\" srcset=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-1.png 799w, https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-1-480x200.png 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 799px, 100vw\" \/><\/p>\n<p><strong>Neuronal network activity characterization of induced neurons (iN) derived from monogenic forms of ASD<\/strong> &#8211; To identify electrical network phenotypes, we exploit the multielectrode-array (MEA) technology (Axion BioSystems) technology and capture key features of neuronal network behavior, such as: activity, synchrony, and network oscillations or bursting. Our final aim is to define a robust electrophysiological feature with relevant translational medicine implication, which will result in an in vitro platform suitable for second-tier hit validation.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-52 aligncenter\" src=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-2.jpg\" alt=\"\" width=\"799\" height=\"333\" srcset=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-2.jpg 799w, https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-2-480x200.jpg 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 799px, 100vw\" \/><\/p>\n<p><strong><em>Development of the in vivo animal models for second tier screening and drug candidate validation<\/em><\/strong><br \/>Our main aim is to define a battery of standard, translationally relevant animal models that will be used for further screening and validation of drug candidates identified <em>in vitro<\/em>. Taking advantage of the mouse models generated by the Seaver Autism Center, the DDD initiative includes performing (1) <em>ex vivo <\/em>electrophysiology assays and (2) in<em> vivo <\/em>electroencephalogram (EEG) measurements in mouse bearing clinically relevant<em> Shank3<\/em>, <em>Ddx3x, Adnp and Foxp1 <\/em>mutations.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-53 aligncenter\" src=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-3.jpg\" alt=\"\" width=\"799\" height=\"333\" srcset=\"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-3.jpg 799w, https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-content\/uploads\/sites\/432\/2022\/06\/Image-3-480x200.jpg 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 799px, 100vw\" \/><\/p>\n<p>[\/et_pb_text][et_pb_divider _builder_version=&#8221;4.9.0&#8243; divider_position=&#8221;center&#8221; divider_weight=&#8221;0.5px&#8221; 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header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; custom_margin=&#8221;0px|||&#8221; custom_padding=&#8221;0px|||&#8221;]<\/p>\n<p><strong>Collaborations<\/strong><\/p>\n<p>[\/et_pb_text][et_pb_text admin_label=&#8221;Collaborations text&#8221; _builder_version=&#8221;4.9.0&#8243; 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header_4_text_shadow_blur_strength=&#8221;header_4_text_shadow_style,%91object Object%93&#8243; header_4_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_5_text_shadow_horizontal_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_5_text_shadow_vertical_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_5_text_shadow_blur_strength=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; text_line_height=&#8221;2em&#8221;]<\/p>\n<p><strong>Rumi Scientific<\/strong><\/p>\n<p><a href=\"https:\/\/www.mountsinai.org\/about\/newsroom\/2021\/mount-sinai-and-rumi-scientific-team-up-to-advance-drug-discovery-for-rare-genetic-disorders-tied-to-autism?_ga=2.14404471.1103363086.1646078400-1267849935.1595883817\">https:\/\/www.mountsinai.org\/about\/newsroom\/2021\/mount-sinai-and-rumi-scientific-team-up-to-advance-drug-discovery-for-rare-genetic-disorders-tied-to-autism?_ga=2.14404471.1103363086.1646078400-1267849935.1595883817<\/a><\/p>\n<p><a href=\"https:\/\/rumiscientific.com\/mount-sinai-and-rumi-scientific-team-up-to-advance-drug-discovery-for-rare-genetic-disorders-tied-to-autism\/\">https:\/\/rumiscientific.com\/mount-sinai-and-rumi-scientific-team-up-to-advance-drug-discovery-for-rare-genetic-disorders-tied-to-autism\/<\/a><\/p>\n<p><strong>ORYZON<\/strong><\/p>\n<p><a href=\"https:\/\/www.oryzon.com\/en\/news-events\/news\/oryzon-starts-preclinical-collaboration-autism-seaver-autism-center-mount-sinai\">https:\/\/www.oryzon.com\/en\/news-events\/news\/oryzon-starts-preclinical-collaboration-autism-seaver-autism-center-mount-sinai<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<p><strong>The Promise of Precision Medicine in Autism<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34293291\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34293291\/<\/a><\/p>\n<p><strong>Screening for Modulators of Autism Spectrum Disorder Using Induced Human Neurons<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35123134\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35123134\/<\/a><\/p>\n<p><strong>Prospective and Detailed Behavioral Phenotyping in DDX3X Syndrome<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33993884\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/33993884\/<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<p><strong>The Promise of Precision Medicine in Autism<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34293291\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34293291\/<\/a><\/p>\n<p><strong>Screening for Modulators of Autism Spectrum Disorder Using Induced Human Neurons<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35123134\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35123134\/<\/a><\/p>\n<p><strong>Prospective and Detailed Behavioral Phenotyping in DDX3X Syndrome<\/strong><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33993884\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/33993884\/<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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custom_padding__hover=&#8221;|||&#8221; custom_padding=&#8221;|||&#8221;][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p><div class=\"et_pb_row et_pb_row_0 et_pb_row_empty\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Our Research The Kostic Lab focuses on drug discovery and development in autism spectrum disorder (ASD). The lab\u2019s main goals are to identify potential drug candidates for treatment of ASD by designing experimental strategies for testing in neuronal cell systems and animal models, as well as to discover and validate molecular biomarkers in ASD. [&hellip;]<\/p>\n","protected":false},"author":274,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-template-blank.php","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-17","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/pages\/17","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/users\/274"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/comments?post=17"}],"version-history":[{"count":41,"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/pages\/17\/revisions"}],"predecessor-version":[{"id":83,"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/pages\/17\/revisions\/83"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/kosticlab\/wp-json\/wp\/v2\/media?parent=17"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}