{"id":12,"date":"2016-08-16T15:27:49","date_gmt":"2016-08-16T15:27:49","guid":{"rendered":"http:\/\/labs.icahn.mssm.edu\/kholab\/?page_id=12"},"modified":"2016-12-12T15:45:03","modified_gmt":"2016-12-12T15:45:03","slug":"research","status":"publish","type":"page","link":"https:\/\/labs.icahn.mssm.edu\/kholab\/research\/","title":{"rendered":"Research"},"content":{"rendered":"

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\nWe have been focusing on identifying and characterizing novel molecular pathways with relevance to human disease. One area of particular interest to us is the role of post-translational modification of key proteins involved in physiological dysfunction in the heart. We developed a number of methodologies for building dynamic protein-protein interaction maps and identified important lysine post-translational modifications of the sarcoplasmic reticulum calcium ATPase (SERCA2a) pump, a key enzyme for muscle relaxation.<\/p>\n

We demonstrated that small ubiquitin-related modifier type 1 (SUMO-1)-mediated modification is a critical post-translational modification that positively regulates SERCA2a function (Kho et al., Nature, 2011). Both murine and swine model studies suggest that increasing SUMO-1 levels may be a new therapeutic strategy to treat heart failure. Furthermore, the potential of this strategy is reinforced by the fact that the novel SUMO activator, N106, is a first-in-class agent with significant effects on myocardial dysfunction (Kho et al., Nature Communications, 2015). We use both in vitro<\/i> proteomic approaches, biochemistry and in vivo<\/i> mouse physiology analyses. Current research projects of my group as follows:<\/p>\n

    \n
  1. Identifying SUMO-1 modified proteome under the physiological and pathological conditions and also identifying new targets for preventive and therapeutic purposes for heart failure.<\/li>\n
  2. Understanding the regulation of SUMO-1 gene by microRNAs in the heart. We are focused now on determining whether microRNA-mediated SUMO-1 regulation would be beneficial in cardiac dysfunction.<\/li>\n
  3. Characterizaing the relationship of post-translational acetylation of SERCA2a in the myocardium and their potential as new molecular pathway unerpinning physiological dysfunction and heart failure.<\/li>\n<\/ol>\n

    This research aims to validate the SUMO pathway and its potential therapeutic target for heart failure.<\/p>\n","protected":false},"excerpt":{"rendered":"

    We have been focusing on identifying and characterizing novel molecular pathways with relevance to human disease. One area of particular interest to us is the role of post-translational modification of key proteins involved in physiological dysfunction in the heart. We developed a number of methodologies for building dynamic protein-protein interaction maps and identified important lysine […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-12","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/pages\/12","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/comments?post=12"}],"version-history":[{"count":3,"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/pages\/12\/revisions"}],"predecessor-version":[{"id":36,"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/pages\/12\/revisions\/36"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/kholab\/wp-json\/wp\/v2\/media?parent=12"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}