{"id":1287,"date":"2017-10-27T10:53:01","date_gmt":"2017-10-27T14:53:01","guid":{"rendered":"http:\/\/labs.icahn.mssm.edu\/garcia-sastre\/?page_id=1287"},"modified":"2019-02-22T12:11:07","modified_gmt":"2019-02-22T17:11:07","slug":"giuseppe-pisanelli-d-v-m-ph-d","status":"publish","type":"page","link":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/giuseppe-pisanelli-d-v-m-ph-d\/","title":{"rendered":"Giuseppe Pisanelli, D.V.M., Ph.D."},"content":{"rendered":"

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Instructor
\n<\/em>giuseppe.pisanelli@mssm.edu<\/a><\/p>\n

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\"Research<\/a><\/p>\n

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Research Interests<\/span>
\n<\/strong>Yellow fever virus (YFV) is the etiologic agent of yellow fever. There are no antivirals available to treat yellow fever, and attempts to develop YFV-specific antivirals or to develop alternative vaccines have been hampered by the lack of an immunocompetent mouse model of yellow fever. It has been reported that both the wild-type (Asibi) strain and the 17D strain of yellow fever infect mice with defects in type I IFN (IFN-I) signaling. This suggests that the IFN-I response serves as the major barrier to mouse infection. We have recently shown that YFV antagonizes IFN-I signaling in human and non-human primate cells and that the NS5 proteins of both YFV-Asibi and YFV-17D bind to and sequester human STAT2 after interferon signaling. My research project\u2019s aim is to identify the host restriction factors responsible for the inability of the YF-NS5 protein to bind to murine STAT2 and inhibit murine IFN-I signaling. This will be potentially useful for the development of a Yellow Fever mouse model.<\/p>\n

Notable Publications
\n<\/strong><\/span>Giuseppe Pisanelli<\/strong>, Maudry Laurent-Rolle, Balaji Manicassamy, Alan Belicha-Villanueva, Juliet Morrison, Bernardo Lozano-Dubernard, Felipa Castro-Peralta, Giuseppe Iovane, and Adolfo Garc\u00eda-Sastre. La Piedad Michoac\u00e1n Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation<\/a>. Virus Research. 2016 Feb 2;213:11-22. doi: 10.1016\/j.virusres.2015.10.027.<\/p>\n

Maudry Laurent-Rolle, Juliet Morrison, Ricardo Rajsbaum, Jesica M. Levingston Macleod, Giuseppe Pisanelli<\/strong>, Alissa Pham, Juan Ayllon, Lisa Miorin, Carles Mart\u00ednez-Romero, Benjamin R Tenoever, and Adolfo Garc\u00eda-Sastre. The interferon signaling antagonist function of yellow fever virus NS5 protein is activated by Type I interferon<\/a>. Cell Host and Microbes. 2014 Sep 10;16(3):314-27. doi: 10.1016\/j.chom.2014.07.015.<\/p>\n

Ricardo Rajsbaum, Gijs\u00a0A. Versteeg, Sonja Schmid, Ana\u00a0M. Maestre, Alan Belicha-Villanueva, Carles Mart\u00ednez-Romero, Jenish\u00a0R. Patel, Juliet Morrison, Giuseppe Pisanelli<\/strong>, Lisa Miorin, Maudry Laurent-Rolle, Hong\u00a0M. Moulton, David\u00a0A. Stein, Ana Fernandez-Sesma, Benjamin\u00a0R. Tenoever, and Adolfo Garc\u00eda-Sastre. Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKK\u03b5 Kinase-Mediated Antiviral Response<\/a>. Immunity. 2014 Jun 19;40(6):880-95. doi: 10.1016\/j.immuni.2014.04.018. Epub 2014 May 29. PMID:\u00a024882218.<\/p>\n

Juliet Morrison, Maudry Laurent-Rolle, Ana Maestre, Giuseppe Pisanelli<\/strong>, Lubbertus C.F. Mulder, Viviana Simon, Ana Fernandez-Sesma, Adolfo Garc\u00eda-Sastre. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I IFN signaling. <\/a>PLOS Pathogens. 2013 Mar;9(3):e1003265. doi: 10.1371\/journal.ppat.1003265. Epub 2013 Mar 28.<\/p>\n

Balaji Manicassamy, Santhakumar Manicassamy, Alan Belicha-Villanueva, Giuseppe Pisanelli<\/strong>, Bali Pulendran And Adolfo Garc\u00eda-Sastre. Analysis of in vivo<\/em> dynamics of influenza virus infection using a GFP reporter virus<\/a>. Proceedings of National Academy of Sciences of\u00a0 the United States of America. 2010 Jun 22;107(25):11531-6. Epub 2010 Jun 7.<\/p>\n","protected":false},"excerpt":{"rendered":"

Instructor giuseppe.pisanelli@mssm.edu           Research Interests Yellow fever virus (YFV) is the etiologic agent of yellow fever. There are no antivirals available to treat yellow fever, and attempts to develop YFV-specific antivirals or to develop alternative vaccines have been hampered by the lack of an immunocompetent mouse model of yellow fever. It […]<\/p>\n","protected":false},"author":36,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1287","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/pages\/1287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/users\/36"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/comments?post=1287"}],"version-history":[{"count":16,"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/pages\/1287\/revisions"}],"predecessor-version":[{"id":1738,"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/pages\/1287\/revisions\/1738"}],"wp:attachment":[{"href":"https:\/\/labs.icahn.mssm.edu\/garcia-sastre\/wp-json\/wp\/v2\/media?parent=1287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}