ES cell culture
References, reagents and conditions for ES cell culture, electroporation and expansion.
Buffer, reaction mix and sample prep for cells and tissue.
PCR screening of ES cell colonies
Reagents and procedure for identification of targeted clones.
Processing gel, transfer, probing and washing, and exposure to film.
Southern blot screening of ES cell colonies
Reference to protocol adapted to 96 well plate format.
X-gal staining of cell lines and embryos
Solutions, volumes and fixation times for cells and embryos.