The main focus of my research is to determine the interaction of host factors during influenza A virus infection. This is a broad field so on one hand we focus on the role of the adaptive mutations within the influenza A virus polymerase in conferring species-specific interactions with essential host factors. From a high-throughput genetic screen we identified three main factors involved in the polymerase adaptation and we are working on the characterization of these factors. On the other hand, from datasets of RNAseq performed from influenza infections in primary cells, we saw an increase on some non-coding RNAs and we are working to try to elucidate the mechanism behind this phenomenon.
Grant Beyleveld, Daniel J. Chin, Elena Moreno Del Olmo, Jade Carter, Isabel Najera, Cristian Cillóniz, Megan L. Shaw. Nucleolar Relocalization of RBM14 by Influenza A Virus NS1 Protein. mSphere Nov 2018, 3 (6) e00549-18; doi: 10.1128/mSphereDirect.00549-18
Moreno E, Gallego I, Gregori J, Lucía-Sanz A, Soria ME, Castro V, Beach NM, Manrubia SC, Quer J, Esteban JI, Rice CM, Gómez J, Gastaminza P, Domingo E and Perales C. Internal disequilibria and phenotypic diversification during replication of hepatitis C virus in a non-coevolving cellular environment. J Virol 2017 Apr 28;91(10) e02505-16. doi: 10.1128/JVI.02505-16.
Moreno E and Perales C. Distance effects during polyprotein processing in the complementation between defective FMDV RNAs. J Gen Virol 2016. 97:1575-1583. doi: 10.1099/jgv.0.000480.
Perales C, Moreno E and Domingo E. Clonality and intracellular polyploidy in virus evolution and pathogenesis. PNAS 2015; 112(29): 8887-8892. doi:10.1073/pnas.1501715112
Moreno E, Ojosnegros S, García-Arriaza J, Escarmís C, Domingo E and Perales C. Exploration of sequence space as the basis of viral RNA genome segmentation. PNAS 2014; 111(18): 6678-6683. doi:10.1073/pnas.1323136111