Stem Cell Services

The Pluripotent Stem Cell Shared Resource facility provides three main types of services: expansion of pluripotent stem cells, the generation of pluripotent stem cells and Training.

1. Expansion of pluripotent stem cell lines – $500 

This includes: 

  • Initial mycoplasma testing and FISH analysis of the samples
    • The facility will not work with any samples that are mycoplasma positive.
  • Expansion of  cells in either feeder or feeder free conditions
  • 10 vials of frozen pluripotent stem cells.
  • Optional end stage analysis – an extra $300
    • Samples will be karyotyped and tested for mycoplasma.

2. Reprograming fibroblast – $2,500 (6-12 clones/line) 

This process can take between 2-3 months.

  • Investigators can either provide us with at least 2 vials of frozen fibroblast cells or at least 3 wells of a 6 well plates. The fibroblasts should be of a low passage. They should be actively proliferating. Cells that have slow proliferation rate do not have a high success rate in being reprogramed. Upon receiving the samples, strategies will be discussed based on the proliferating rate of the fibroblast. If a new sample can not be obtained and investigator wishes to proceed forward with the reprograming, the investigator will bear the whole cost regardless of the outcome. 
  • Initial Mycoplasma testing
    • The facility will only accept mycoplasma negative cells, please provide documentation of this when dropping of cells. We will repeat the test in the facility as well.
  • A choice between using Sendai Virus or mRNA reprograming will be made
  • Cells will be reprogramed
  • 6-12 – Tra-1-81 positive colonies will be picked
    • At this point, if the investigator is with in Mount Sinai, they will be requested to come pick up the live culture. If the investigator is from a different institute, they will be mailed frozen colonies.
    • At the time of the hand over, the investigator may decide to have the facility expand and freeze 3-6 colonies as a ‘back up’ for an additional $500

Sometimes, for no apparent reason, fibroblast do not reprogram. In this case we will try a second attempt at reprogramming with altered strategies. At this time a price will be re-negotiated 

3. Reprograming Blood using Sendai Virus  – $4,060/sample & $450/clone 

  • Peripheral blood mononuclear cells (PBMC) will be isolated from atleast 6 mL of bloodThe following is the breakdown of costs/sample
    # Scope of work Cost/sample
    1 Expansion of erythroblasts $800
    2 Mycoplasma testing $50
    3 XY FISH $60
    4 FACS on pre infected erythroblasts $50/sample
    5 SeV infection – upto iPSC colony picking $3000/sample
    Tra-1-81 staining on iPSC colonies $100/sample
    6 Expansion of 3 clones upto SeV-free stage- 2 months- 6-8 passages- 3 clones 350/clone
    7 Freezing at every other passage upto passage 8 (2 vials) $100/clone
  • At the time of the hand over, the investigator may decide to have the facility expand and freeze 3-6 colonies as a ‘back up’ for an additional $500

Additional work can be purchased at the following costs:

1 Karyotyping $300/clone
2 Immunohistochemistry $200/clone
3 3-layer EB differentiation $1000/clone
4 Fingerprinting TBD

Sometimes, for no apparent reason, fibroblast do not reprogram. In this case we will try a second attempt at reprogramming with altered strategies. At this time a price will be re negotiated  

4. Training 

Extensive Course – $750 

The core offers an extensive 1 month course every 3 months. Ideally a single lab member should attend this course and train subsequent lab members. This course includes:

  • Basic culture techniques for pluripotent stem cells 
    • Media Constitution
    • Passaging cells
    • Thawing cells
    • Freezing cells
  • Trouble Shooting
    • Removal of differentiated cells using a variety of techniques
  • Colony picking
  • Immunostaining
  • Preparing sample for FACS analysis

Please note that you must attend the FACs training to use their facility 

Differentiation Course – $750 

This course will be a specialized course where the trainee will differentiate pluripotent stem cells into one of the three germ layers.

Quick Refresher Course – $250 

This is a 1 week course for those with prior training who may need a refresher on current techniques and help with trouble shooting.